Abstract

Specific differentiated cell types in culture will be developed and exploited to study the normal physiology and metabolic disorders of parent tissues. The nutritive, hormonal and extracellular matrix requirements for growth and function of several differentiated cell types have been or will be optimized. Bioeffectors, receptors, transducers and gene products will be purified and characterized by techniques of protein chemistry and molecular biology. Immunochemical and cDNA probes will be developed to study regulation of cell growth and specific gene products by hormones. Our basic premise is that factors discovered in culture that affect growth, function and differentiation are hormones involved in tissue physiology. The elucidation of these factors, their physiologic action and mechanism of action have practical importance in the management of metabolic disorders. The program consists of five projects devoted to these problems as well as a protein chemistry and molecular biology core support laboratories.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK)
Type
Research Program Projects (P01)
Project #
5P01DK038639-04
Application #
3095504
Study Section
Diabetes, Endocrinology and Metabolic Diseases B Subcommittee (DDK)
Project Start
1988-03-01
Project End
1993-02-28
Budget Start
1991-03-01
Budget End
1992-02-29
Support Year
4
Fiscal Year
1991
Total Cost
Indirect Cost

  Institution

Name
Adirondack Biomedical Research Institute
Department
Type
DUNS #
City
Lake Placid
State
NY
Country
United States
Zip Code
12946

  Publications

Yu, Y; Sato, J D (1999) MAP kinases, phosphatidylinositol 3-kinase, and p70 S6 kinase mediate the mitogenic response of human endothelial cells to vascular endothelial growth factor. J Cell Physiol 178:235-46
Herley, M T; Yu, Y; Whitney, R G et al. (1999) Characterization of the VEGF binding site on the Flt-1 receptor. Biochem Biophys Res Commun 262:731-8
Liu, P; Lalor, D; Bowser, S S et al. (1998) Regulation of arachidonic acid release and prostaglandin E2 production in thymic epithelial cells by ATPgammaS and transforming growth factor-alpha. Cell Immunol 188:81-8
Ye, H; Serrero, G (1998) Stimulation of adipose differentiation related protein (ADRP) expression by ibuprofen and indomethacin in adipocyte precursors and in adipocytes. Biochem J 330 ( Pt 2):803-9
Serrero, G; Lepak, N M (1997) Prostaglandin F2alpha receptor (FP receptor) agonists are potent adipose differentiation inhibitors for primary culture of adipocyte precursors in defined medium. Biochem Biophys Res Commun 233:200-2
Kapron, J T; Hilliard, G M; Lakins, J N et al. (1997) Identification and characterization of glycosylation sites in human serum clusterin. Protein Sci 6:2120-33
Myoken, Y; Myoken, Y; Okamoto, T et al. (1997) Immunohistochemical localization of fibroblast growth factor-1 (FGF-1), FGF-2 and fibroblast growth factor receptor-1 (FGFR-1) in pleomorphic adenoma of the salivary glands. J Oral Pathol Med 26:17-22
Matsuzaki, K; Kan, M; McKeehan, W L (1996) Reconstitution of a pentameric complex of dimeric transforming growth factor beta ligand and a type I, II, III receptor in baculoviral-infected insect cells. In Vitro Cell Dev Biol Anim 32:345-60
Feng, S; Xu, J; Wang, F et al. (1996) Nuclear localization of a complex of fibroblast growth factor(FGF)-1 and an NH2-terminal fragment of FGF receptor isoforms R4 and R1alpha in human liver cells. Biochim Biophys Acta 1310:67-73
Luo, Y; Gabriel, J L; Wang, F et al. (1996) Molecular modeling and deletion mutagenesis implicate the nuclear translocation sequence in structural integrity of fibroblast growth factor-1. J Biol Chem 271:26876-83

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