The recombinant DNA laboratory will serve as a central resource in the program for obtaining information and materials related to new growth factors which will be purified from a variety of tissue culture systems of normal cells. The types of projects that will be served have a common element in that there is an unknown factor or factors either produced by cells or contained in crude extracts of tissues such as brain, which have a profound effect on the growth properties of those cells under study. As these new growth factors are purified the recombinant DNA laboratory will prepare complementary DNA libraries to total poly(A+)-RNA isolated from the cell lines under study in this program project. It will be fairly straightforward to prepare cloned cDNA libraries with 10/6 to 10/7 clones per mug of poly(A+)-RNA. This should be sufficient to find the cDNA to a given messenger RNA which is in a concentration as low as 1 part in 10/7 to the total messenger RNA. Once a cDNA to a new growth factor is identified, the complete nucleotide sequence of the messenger RNA can be determined. The cDNA libraries will mainly be prepared in the lambda vector lambda gt 11 or 10. Two of these vectors will allow high level expression of fused proteins coded for by these messenger RNAs. These various clones can then be screened by using antibody to the new factor, or as amino acid information becomes available on the new factors, specific oligonucleotide probes can be synthesized to screen and isolate the appropriate clones from the cDNA libraries. Finally, and most importantly, the cDNAs to the new growth factors will be used to analyze the expression of these genes in normal and abnormal tissues and cells in vitro which then may shed light on the role of these factors in normal abnormal cellular physiology both in vitro and in vivo.

Project Start
Project End
Budget Start
Budget End
Support Year
5
Fiscal Year
1992
Total Cost
Indirect Cost
Name
Adirondack Biomedical Research Institute
Department
Type
DUNS #
City
Lake Placid
State
NY
Country
United States
Zip Code
12946
Yu, Y; Sato, J D (1999) MAP kinases, phosphatidylinositol 3-kinase, and p70 S6 kinase mediate the mitogenic response of human endothelial cells to vascular endothelial growth factor. J Cell Physiol 178:235-46
Herley, M T; Yu, Y; Whitney, R G et al. (1999) Characterization of the VEGF binding site on the Flt-1 receptor. Biochem Biophys Res Commun 262:731-8
Liu, P; Lalor, D; Bowser, S S et al. (1998) Regulation of arachidonic acid release and prostaglandin E2 production in thymic epithelial cells by ATPgammaS and transforming growth factor-alpha. Cell Immunol 188:81-8
Ye, H; Serrero, G (1998) Stimulation of adipose differentiation related protein (ADRP) expression by ibuprofen and indomethacin in adipocyte precursors and in adipocytes. Biochem J 330 ( Pt 2):803-9
Serrero, G; Lepak, N M (1997) Prostaglandin F2alpha receptor (FP receptor) agonists are potent adipose differentiation inhibitors for primary culture of adipocyte precursors in defined medium. Biochem Biophys Res Commun 233:200-2
Kapron, J T; Hilliard, G M; Lakins, J N et al. (1997) Identification and characterization of glycosylation sites in human serum clusterin. Protein Sci 6:2120-33
Myoken, Y; Myoken, Y; Okamoto, T et al. (1997) Immunohistochemical localization of fibroblast growth factor-1 (FGF-1), FGF-2 and fibroblast growth factor receptor-1 (FGFR-1) in pleomorphic adenoma of the salivary glands. J Oral Pathol Med 26:17-22
Myoken, Y; Myoken, Y; Okamoto, T et al. (1996) Expression of fibroblast growth factor-1 (FGF-1), FGF-2 and FGF receptor-1 in a human salivary-gland adenocarcinoma cell line: evidence of growth. Int J Cancer 65:650-7
Serrero, G; Lepak, N (1996) Endocrine and paracrine negative regulators of adipose differentiation. Int J Obes Relat Metab Disord 20 Suppl 3:S58-64
Matsuzaki, K; Kan, M; McKeehan, W L (1996) Reconstitution of a pentameric complex of dimeric transforming growth factor beta ligand and a type I, II, III receptor in baculoviral-infected insect cells. In Vitro Cell Dev Biol Anim 32:345-60

Showing the most recent 10 out of 65 publications