The Biological Services Core laboratory of the BDC provides support in six areas for the Program Project investigators. These operations are supervised by Dr. K. Haskins and are managed by Philip Pratt. The Biological Services Core lab contains a CryoMed Liquid Nitrogen Cryopreservation System, for freezing and storing cells and tissues, which will be used in projects 2,3,4 and 6. The Graphics section supplies all the researchers with photographic prints and transparencies, and with both free-hand and computer-generated graphs, charts and illustrations. A fully functional photographic dark room has just been completed at the BDC. Biological Services maintains the non-obese diabetic (NOD) mouse inbred breeding colony for use in Projects 2 through 6. The primary breeding line is housed under sterile conditions within one room of the BDC animal facility. A second room holds the stock NOD's and the breeding sublines. The diabetic status of each animal is routinely monitored and diabetic animals are maintained by insulin therapy. Three technical assistants within Biological Services routinely isolate (collagenase digestion and microscopic harvesting) and culture rat and mouse pancreatic islets for use by investigators in Project 2 and 3. Several part-time student workers routinely wash and sterilize glassware for Projects 1,2,3,4 and 6. Lastly, Biological Services contains the BDC histology/immunohistology section which is fully equipped to provide processing, embedding, cutting and staining of both paraffin-embedded and frozen tissues for all the Project's researchers.

Project Start
Project End
Budget Start
Budget End
Support Year
4
Fiscal Year
1991
Total Cost
Indirect Cost
Name
University of Colorado Denver
Department
Type
DUNS #
065391526
City
Aurora
State
CO
Country
United States
Zip Code
80045
Peterson, J D; Haskins, K (1996) Transfer of diabetes in the NOD-scid mouse by CD4 T-cell clones. Differential requirement for CD8 T-cells. Diabetes 45:328-36
Sellins, K S; Gold, D P; Bellgrau, D (1996) Resistance to tolerance induction in the diabetes-prone biobreeding rat as one manifestation of abnormal responses to superantigens. Diabetologia 39:28-36
Gold, D P; Shaikewitz, S T; Mueller, D et al. (1995) T cells from BB-DP rats show a unique cytokine mRNA profile associated with the IDDM1 susceptibility gene, Lyp. Autoimmunity 22:149-61
Peterson, J D; Pike, B; Dallas-Pedretti, A et al. (1995) Induction of diabetes with islet-specific T-cell clones is age dependent. Immunology 85:455-60
Bergman, B; Haskins, K (1994) Islet-specific T-cell clones from the NOD mouse respond to beta-granule antigen. Diabetes 43:197-203
Peterson, J D; Pike, B; McDuffie, M et al. (1994) Islet-specific T cell clones transfer diabetes to nonobese diabetic (NOD) F1 mice. J Immunol 153:2800-6
Bellgrau, D; Redd, J M; Sellins, K S (1994) Peculiar T-cell signaling does not preclude positive selection in the diabetes-prone BB rat. Diabetes 43:47-52
Shehadeh, N N; LaRosa, F; Lafferty, K J (1993) Altered cytokine activity in adjuvant inhibition of autoimmune diabetes. J Autoimmun 6:291-300
Lafferty, K J; Gill, R G (1993) The maintenance of self-tolerance. Immunol Cell Biol 71 ( Pt 3):209-14
Shehadeh, N N; Gill, R G; Lafferty, K J (1993) Mechanism of self-tolerance to endocrine tissue. Springer Semin Immunopathol 14:203-20

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