Gene Expression Core

Magnuson, M A.

Abstract

The Gene Expression Core is designed to provide the following services to the group. (a) Oligonucleotides will be synthesized upon request. They will be purified and checked for purity. The sequences of the oligonucleotides will be automatically checked with our database for sequence identity with (i) other oligonucleotides ordered through the core and (ii) with known DNA/protein binding sequences. (b) We propose to supply additional core instrumentation designed to facilitate both extract purification and plasmid and virus preparations. In addition instrumentation will be obtained to support transient transfection analysis, cloning and screening and also database searching. (c) The core will maintain a large inventory of enzymes (this will be integrated with the ongoing DRTC-supported DNA core), as well as kits, expression vectors, libraries and bacterial/yeast strains. In addition we propose to accumulate a stock of identified DNA-binding proteins for comparison purposes. (d) The core will prepare transcription extracts, as well as perform large-scale cell preparations for factor isolation purposes.

Funding Agency

• Agency: National Institute of Health (NIH)
• Institute: National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK)
• Type: Research Program Projects (P01)
• Project #: 5P01DK042502-02
• Application #: 3855259
• Support Year: 2
• Fiscal Year: 1991

Institution

• Name: Vanderbilt University Medical Center
• DUNS #: 004413456
• City: Nashville
• State: TN
• Country: United States
• Zip Code: 37212

Publications

Zhang, Juliet; Weinrich, Jarret A P; Russ, Jeffrey B et al. (2017) A Role for Dystonia-Associated Genes in Spinal GABAergic Interneuron Circuitry. Cell Rep 21:666-678

Krah, Nathan M; De La O, Jean-Paul; Swift, Galvin H et al. (2015) The acinar differentiation determinant PTF1A inhibits initiation of pancreatic ductal adenocarcinoma. Elife 4:

Baeyens, Luc; Lemper, Marie; Leuckx, Gunter et al. (2014) Transient cytokine treatment induces acinar cell reprogramming and regenerates functional beta cell mass in diabetic mice. Nat Biotechnol 32:76-83

Liu, Jing; Willet, Spencer G; Bankaitis, Eric D et al. (2013) Non-parallel recombination limits Cre-LoxP-based reporters as precise indicators of conditional genetic manipulation. Genesis 51:436-42

Kopinke, Daniel; Brailsford, Marisa; Pan, Fong Cheng et al. (2012) Ongoing Notch signaling maintains phenotypic fidelity in the adult exocrine pancreas. Dev Biol 362:57-64

Gu, Yanyun; Lindner, Jill; Kumar, Anil et al. (2011) Rictor/mTORC2 is essential for maintaining a balance between beta-cell proliferation and cell size. Diabetes 60:827-37

Pound, Lynley D; Hang, Yan; Sarkar, Suparna A et al. (2011) The pancreatic islet ?-cell-enriched transcription factor Pdx-1 regulates Slc30a8 gene transcription through an intronic enhancer. Biochem J 433:95-105

Papizan, James B; Singer, Ruth A; Tschen, Shuen-Ing et al. (2011) Nkx2.2 repressor complex regulates islet ?-cell specification and prevents ?-to-?-cell reprogramming. Genes Dev 25:2291-305

Artner, Isabella; Hang, Yan; Mazur, Magdalena et al. (2010) MafA and MafB regulate genes critical to beta-cells in a unique temporal manner. Diabetes 59:2530-9

Masui, Toshihiko; Swift, Galvin H; Deering, Tye et al. (2010) Replacement of Rbpj with Rbpjl in the PTF1 complex controls the final maturation of pancreatic acinar cells. Gastroenterology 139:270-80