Abstract

The application of methods for retroviral mediated gene transfer and hepatocellular cultivation to hepatic gene therapy requires large scale production of retroviruses and large scale cultivation of hepatocytes. The """"""""Virus and Tissue Culture Core"""""""" is designed to provide retroviruses, to provide facilities for large scale preparation of hepatocytes, and provide hepatocyte feeder stocks and cultures is association with the experiments of the investigators. Each of the investigators participating in this program project grant will make use of retroviruses and each investigator will make use of hepatocyte cultures. This core play a particularly active role in the procurement as well as the harvesting of human liver samples which will be used by several investigators on this project.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK)
Type
Research Program Projects (P01)
Project #
5P01DK044080-03
Application #
3776727
Study Section
Project Start
Project End
Budget Start
Budget End
Support Year
3
Fiscal Year
1993
Total Cost
Indirect Cost

  Institution

Name
Baylor College of Medicine
Department
Type
DUNS #
074615394
City
Houston
State
TX
Country
United States
Zip Code
77030

  Publications

Tarlow, Branden D; Pelz, Carl; Naugler, Willscott E et al. (2014) Bipotential adult liver progenitors are derived from chronically injured mature hepatocytes. Cell Stem Cell 15:605-18
Wu, Y; Teng, B B; Brandt, M L et al. (1999) Normal perinatal rise in serum cholesterol is inhibited by hepatic delivery of adenoviral vector expressing apolipoprotein B mRNA editing enzyme (Apobec1) in rabbits. J Surg Res 85:148-57
Muzzin, P; Eisensmith, R C; Copeland, K C et al. (1997) Hepatic insulin gene expression as treatment for type 1 diabetes mellitus in rats. Mol Endocrinol 11:833-7
Kuzmin, A I; Finegold, M J; Eisensmith, R C (1997) Macrophage depletion increases the safety, efficacy and persistence of adenovirus-mediated gene transfer in vivo. Gene Ther 4:309-16
Fang, B; Wang, H; Gordon, G et al. (1996) Lack of persistence of E1- recombinant adenoviral vectors containing a temperature-sensitive E2A mutation in immunocompetent mice and hemophilia B dogs. Gene Ther 3:217-22
Brandt, M L (1996) Gene therapy in pediatric surgery. Semin Pediatr Surg 5:197-205
Bowles, N E; Eisensmith, R C; Mohuiddin, R et al. (1996) A simple and efficient method for the concentration and purification of recombinant retrovirus for increased hepatocyte transduction in vivo. Hum Gene Ther 7:1735-42
Brandt, M L; Eckert, J W; Buerkle, C J et al. (1996) The subcutaneous spleen: a new model for percutaneous access to the portal venous system. J Invest Surg 9:161-6
Eckert, J W; Buerkle, C J; Major, A M et al. (1995) In situ hybridization utilizing a Y chromosome DNA probe. Use as a cell marker for hepatocellular transplantation. Transplantation 59:109-11
Fang, B; Eisensmith, R C; Wang, H et al. (1995) Gene therapy for hemophilia B: host immunosuppression prolongs the therapeutic effect of adenovirus-mediated factor IX expression. Hum Gene Ther 6:1039-44

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