Abstract

application): The goals of the cell culture/transfection core are to provide proven cell culture and transfection methodology and stocks of well-characterized WIF-B and MDCK lines to PPG users. There are several advantages to making these services part of a central core that is available to multiple users. Stocks of valuable epithelial cell clones can be characterized, maintained and stored for use by all PPG labs and distribution to those outside the PPG. This insures a uniform starting point for all PPG projects using a particular line and fosters interactions among the projects and sharing of expertise. Development of efficient and effective gene delivery systems for expression of foreign proteins in polarized epithelial cells can be optimized in one facility through the cooperation of investigators with different backgrounds. Development time for new cell manipulation techniques that will be used in multiple projects is minimized through reciprocal interactions of the PPG labs with Core B.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK)
Type
Research Program Projects (P01)
Project #
5P01DK044375-10
Application #
6564276
Study Section
Project Start
2002-01-01
Project End
2002-12-31
Budget Start
Budget End
Support Year
10
Fiscal Year
2002
Total Cost
$146,667
Indirect Cost

  Institution

Name
Johns Hopkins University
Department
Type
DUNS #
045911138
City
Baltimore
State
MD
Country
United States
Zip Code
21218

  Publications

Suzuki, Kenichi G N; Fujiwara, Takahiro K; Edidin, Michael et al. (2007) Dynamic recruitment of phospholipase C gamma at transiently immobilized GPI-anchored receptor clusters induces IP3-Ca2+ signaling: single-molecule tracking study 2. J Cell Biol 177:731-42
Suzuki, Kenichi G N; Fujiwara, Takahiro K; Sanematsu, Fumiyuki et al. (2007) GPI-anchored receptor clusters transiently recruit Lyn and G alpha for temporary cluster immobilization and Lyn activation: single-molecule tracking study 1. J Cell Biol 177:717-30
Brown, Christa L; Maier, Kerstin C; Stauber, Tobias et al. (2005) Kinesin-2 is a motor for late endosomes and lysosomes. Traffic 6:1114-24
Berezuk, Matthew A; Schroer, Trina A (2004) Fractionation and characterization of kinesin II species in vertebrate brain. Traffic 5:503-13
Nyasae, Lydia K; Hubbard, Ann L; Tuma, Pamela L (2003) Transcytotic efflux from early endosomes is dependent on cholesterol and glycosphingolipids in polarized hepatic cells. Mol Biol Cell 14:2689-705
Tuma, Pamela L; Hubbard, Ann L (2003) Transcytosis: crossing cellular barriers. Physiol Rev 83:871-932
King, Stephen J; Brown, Christa L; Maier, Kerstin C et al. (2003) Analysis of the dynein-dynactin interaction in vitro and in vivo. Mol Biol Cell 14:5089-97
Bastaki, M; Braiterman, L T; Johns, D C et al. (2002) Absence of direct delivery for single transmembrane apical proteins or their ""Secretory"" forms in polarized hepatic cells. Mol Biol Cell 13:225-37
Tuma, Pamela L; Nyasae, Lydia K; Hubbard, Ann L (2002) Nonpolarized cells selectively sort apical proteins from cell surface to a novel compartment, but lack apical retention mechanisms. Mol Biol Cell 13:3400-15
Quintyne, Nicholas J; Schroer, Trina A (2002) Distinct cell cycle-dependent roles for dynactin and dynein at centrosomes. J Cell Biol 159:245-54

Showing the most recent 10 out of 36 publications