Core - Biological Resources

Kwok, William W.

Abstract

Core B will prepare, store, and distribute cells, DNA, synthetic oligonucleotides, and peptides to the program project investigators and coordinate access to the FACScan for flow cytometry. Core B will also maintain the central core data bank with clinical and laboratory data bases.

Funding Agency

• Agency: National Institute of Health (NIH)
• Institute: National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK)
• Type: Research Program Projects (P01)
• Project #: 5P01DK049841-02
• Application #: 5210933
• Support Year: 2
• Fiscal Year: 1996

Publications

LaGasse, James M; Brantley, Michael S; Leech, Nicola J et al. (2002) Successful prospective prediction of type 1 diabetes in schoolchildren through multiple defined autoantibodies: an 8-year follow-up of the Washington State Diabetes Prediction Study. Diabetes Care 25:505-11

Nepom, G T; Lippolis, J D; White, F M et al. (2001) Identification and modulation of a naturally processed T cell epitope from the diabetes-associated autoantigen human glutamic acid decarboxylase 65 (hGAD65). Proc Natl Acad Sci U S A 98:1763-8

Ettinger, R A; Liu, A W; Nepom, G T et al. (2000) Beta 57-Asp plays an essential role in the unique SDS stability of HLA-DQA1*0102/DQB1*0602 alpha beta protein dimer, the class II MHC allele associated with protection from insulin-dependent diabetes mellitus. J Immunol 165:3232-8

Kwok, W W; Liu, A W; Novak, E J et al. (2000) HLA-DQ tetramers identify epitope-specific T cells in peripheral blood of herpes simplex virus type 2-infected individuals: direct detection of immunodominant antigen-responsive cells. J Immunol 164:4244-9

McFarland, B J; Sant, A J; Lybrand, T P et al. (1999) Ovalbumin(323-339) peptide binds to the major histocompatibility complex class II I-A(d) protein using two functionally distinct registers. Biochemistry 38:16663-70

Reichstetter, S; Kwok, W W; Nepom, G T (1999) Impaired binding of a DQ2 and DQ8-binding HSV VP16 peptide to a DQA1*0501/DQB1*0302 trans class II heterodimer. Tissue Antigens 53:101-5

Huang, S C; Glas, A M; Pinchuk, G V et al. (1999) Human B cells accumulate immunoglobulin V gene somatic mutations in a cell contact-dependent manner in cultures supported by activated T cells but not in cultures supported by CD40 ligand. Clin Exp Immunol 116:441-8

Reijonen, H; Elliott, J F; van Endert, P et al. (1999) Differential presentation of glutamic acid decarboxylase 65 (GAD65) T cell epitopes among HLA-DRB1*0401-positive individuals. J Immunol 163:1674-81

Reichstetter, S; Kwok, W W; Kochik, S et al. (1999) MHC-peptide ligand interactions establish a functional threshold for antigen-specific T cell recognition. Hum Immunol 60:608-18

Sanjeevi, C B; DeWeese, C; Landin-Olsson, M et al. (1997) Analysis of critical residues of HLA-DQ6 molecules in insulin-dependent diabetes mellitus. Tissue Antigens 50:61-5