The function of this Core will be to provide support for the Program in studies requiring the use of animals. Each project has extensive animal requirements for the development and testing of the proposed molecular therapies. Each strategy also must be tested in a suitable animal model following in vitro evaluation. These tests in our animal models will provide the needed efficacy and toxicity data required before proposed therapies can be applied to clinical studies.
The specific aims are to breed and provide currently available transgenic animals; to generate new transgenic mice expressing ribozymes in kidney (TGFbeta1, bFGF); to provide rats and mice for in vivo studies; and to provide tissues for cell culture and protein purification. The Core will also assist in the development of the various gene therapy strategies by supporting in vivo testing, phenotype assessment, and biochemical and histological analyses. One of the primary goals of the Core will be the development transgenic lines and breeding studies between transgenics. The transgenic mice will be created using standard pro-nuclear microinjection with non-homologous recombination. The Core will also provide the technical expertise required for the successful completion of several of the experiments requiring survival surgical procedures including minipump implantation, intra renal artery injections, ureteral injections, and renal clearance studies.
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| Leal-Pinto, Edgar; Teixeira, Avelino; Tran, Baohuong et al. (2005) Presence of the nucleic acid channel in renal brush-border membranes: allosteric modulation by extracellular calcium. Am J Physiol Renal Physiol 289:F97-106 |
| Dutheil, Nathalie; Yoon-Robarts, Miran; Ward, Peter et al. (2004) Characterization of the mouse adeno-associated virus AAVS1 ortholog. J Virol 78:8917-21 |
| Marras, Daniele; Bruggeman, Leslie A; Gao, Feng et al. (2002) Replication and compartmentalization of HIV-1 in kidney epithelium of patients with HIV-associated nephropathy. Nat Med 8:522-6 |
| Schwartz, Elissa J; Fierer, Daniel S; Neumann, Avidan U et al. (2002) HIV-1 dynamics in haemodialysis patients. AIDS 16:1301-3 |
| Schwartz, Elissa J; Neumann, Avidan U; Teixeira, Avelino V et al. (2002) Effect of target cell availability on HIV-1 production in vitro. AIDS 16:341-5 |
| Gusella, G Luca; Fedorova, Elena; Marras, Daniele et al. (2002) In vivo gene transfer to kidney by lentiviral vector. Kidney Int 61:S32-6 |
| Stern, Aaron S; Klotman, Mary E; Ioannou, Yiannis A et al. (2002) Polarity of alpha-galactosidase A uptake by renal tubule cells. Kidney Int 61:S52-5 |
| Gusella, G Luca; Fedorova, Elena; Hanss, Basil et al. (2002) Lentiviral gene transduction of kidney. Hum Gene Ther 13:407-14 |
| Walewski, J L; Keller, T R; Stump, D D et al. (2001) Evidence for a new hepatitis C virus antigen encoded in an overlapping reading frame. RNA 7:710-21 |
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