Since all four Projects of this Program Project center around studies of uroplakins and their genes, they all need to use, repeatedly, a common set of reagents including purified urothelial plaques, plasmids containing uroplakin cDNAs and genes, antibodies to uroplakins, PCR primers for the amplification of uroplakin eDNA and genes, and cultured bovine urothelial cells. Obviously it would not be economical for each laboratory to generate and characterize all these reagents themselves. The purpose of this Molecular Biology Core is therefore to form a central supply of these important research tools that can be shared by every investigator in the Program Project. This offers several major advantages including cost-effectiveness, reproducible quality, ease of comparing data generated by separate Projects, and promotion of interactions among the PIs. In the following sections, we will describe the generation of these reagents, quality control, database, personnel, budget and its justification, the contribution of this Core to the individual Projects, and prioritization of usage. These have remained basically unchanged from the original application, with only minor modifications and updates. This Core has worked extremely well, as evidenced in the Progress Report (section D).

Agency
National Institute of Health (NIH)
Institute
National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK)
Type
Research Program Projects (P01)
Project #
5P01DK052206-10
Application #
7662284
Study Section
Special Emphasis Panel (ZDK1)
Project Start
Project End
Budget Start
2008-07-01
Budget End
2009-06-30
Support Year
10
Fiscal Year
2008
Total Cost
$84,288
Indirect Cost
Name
New York University
Department
Type
DUNS #
121911077
City
New York
State
NY
Country
United States
Zip Code
10016
Chicote, Javier U; DeSalle, Rob; Segarra, José et al. (2017) The Tetraspanin-Associated Uroplakins Family (UPK2/3) Is Evolutionarily Related to PTPRQ, a Phosphotyrosine Phosphatase Receptor. PLoS One 12:e0170196
Norsworthy, Allison N; Pearson, Melanie M (2017) From Catheter to Kidney Stone: The Uropathogenic Lifestyle of Proteus mirabilis. Trends Microbiol 25:304-315
Wankel, Bret; Ouyang, Jiangyong; Guo, Xuemei et al. (2016) Sequential and compartmentalized action of Rabs, SNAREs, and MAL in the apical delivery of fusiform vesicles in urothelial umbrella cells. Mol Biol Cell 27:1621-34
Schaffer, Jessica N; Norsworthy, Allison N; Sun, Tung-Tien et al. (2016) Proteus mirabilis fimbriae- and urease-dependent clusters assemble in an extracellular niche to initiate bladder stone formation. Proc Natl Acad Sci U S A 113:4494-9
Kisiela, Dagmara I; Avagyan, Hovhannes; Friend, Della et al. (2015) Inhibition and Reversal of Microbial Attachment by an Antibody with Parasteric Activity against the FimH Adhesin of Uropathogenic E. coli. PLoS Pathog 11:e1004857
Liu, Yan; Mémet, Sylvie; Saban, Ricardo et al. (2015) Dual ligand/receptor interactions activate urothelial defenses against uropathogenic E. coli. Sci Rep 5:16234
Hickling, Duane R; Sun, Tung-Tien; Wu, Xue-Ru (2015) Anatomy and Physiology of the Urinary Tract: Relation to Host Defense and Microbial Infection. Microbiol Spectr 3:
Vieira, Neide; Deng, Fang-Ming; Liang, Feng-Xia et al. (2014) SNX31: a novel sorting nexin associated with the uroplakin-degrading multivesicular bodies in terminally differentiated urothelial cells. PLoS One 9:e99644
Desalle, Rob; Chicote, Javier U; Sun, Tung-Tien et al. (2014) Generation of divergent uroplakin tetraspanins and their partners during vertebrate evolution: identification of novel uroplakins. BMC Evol Biol 14:13
Mathai, John C; Zhou, Enhua H; Yu, Weiqun et al. (2014) Hypercompliant apical membranes of bladder umbrella cells. Biophys J 107:1273-9

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