Abstract

The Protein Purification Core will provide support to all projects by providing facilities and skills for isolating and purifying native and recombinant proteins. Raw materials for such preparations will be provided by the individual projects, along with suggested methods for the isolations. Protein isolations will be carried out the senior Core technician (50% effort), with supervision by Core leader (5% effort). A Pharmacia AKTA explorer Protein Purification System is requested to assume efficient use of time and materials. Purified proteins will by characterized by spectrophotometry, SDS-gel electrophoresis and, in special cases, by EPR spectroscopy. The Core will serve all projects, with heaviest use by Project 1 (Mechanisms of Cellular Iron Uptake From Transferrin, Project 2 (Functional Domains of the Transferrin Receptor), and Project 5 (Structure, Function and Dynamics in Transferrin), and less demanding use by Project 3 (Uptake of Transferrin and Non- transferrin Bound Fe by SFT) and Project 4 (Role of Copper in Iron Metabolism).

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK)
Type
Research Program Projects (P01)
Project #
1P01DK055495-01
Application #
6105926
Study Section
Project Start
1999-05-01
Project End
2000-04-30
Budget Start
1998-10-01
Budget End
1999-09-30
Support Year
1
Fiscal Year
1999
Total Cost
Indirect Cost

  Institution

Name
Albert Einstein College of Medicine
Department
Type
DUNS #
009095365
City
Bronx
State
NY
Country
United States
Zip Code
10461

  Publications

Ikuta, Katsuya; Yersin, Alexandre; Ikai, Atsushi et al. (2010) Characterization of the interaction between diferric transferrin and transferrin receptor 2 by functional assays and atomic force microscopy. J Mol Biol 397:375-84
Ikuta, Katsuya; Zak, Olga; Aisen, Philip (2004) Recycling, degradation and sensitivity to the synergistic anion of transferrin in the receptor-independent route of iron uptake by human hepatoma (HuH-7) cells. Int J Biochem Cell Biol 36:340-52
Robb, Aeisha; Wessling-Resnick, Marianne (2004) Regulation of transferrin receptor 2 protein levels by transferrin. Blood 104:4294-9
Aisen, Philip (2004) Transferrin receptor 1. Int J Biochem Cell Biol 36:2137-43
Robb, Aeisha D; Ericsson, Maria; Wessling-Resnick, Marianne (2004) Transferrin receptor 2 mediates a biphasic pattern of transferrin uptake associated with ligand delivery to multivesicular bodies. Am J Physiol Cell Physiol 287:C1769-75
Brown, Jing Xu; Buckett, Peter D; Wessling-Resnick, Marianne (2004) Identification of small molecule inhibitors that distinguish between non-transferrin bound iron uptake and transferrin-mediated iron transport. Chem Biol 11:407-16
Navati, Mahantesh S; Samuni, Uri; Aisen, Philip et al. (2003) Binding and release of iron by gel-encapsulated human transferrin: evidence for a conformational search. Proc Natl Acad Sci U S A 100:3832-7
Zak, Olga; Ikuta, Katsuya; Aisen, Philip (2002) The synergistic anion-binding sites of human transferrin: chemical and physiological effects of site-directed mutagenesis. Biochemistry 41:7416-23
Ray, Anandhi; Friedman, Benjamin A; Friedman, Joel M (2002) Trehalose glass-facilitated thermal reduction of metmyoglobin and methemoglobin. J Am Chem Soc 124:7270-1
Han, Okhee; Wessling-Resnick, Marianne (2002) Copper repletion enhances apical iron uptake and transepithelial iron transport by Caco-2 cells. Am J Physiol Gastrointest Liver Physiol 282:G527-33

Showing the most recent 10 out of 13 publications