Since the measurement of peptide hormones and circulating substrates will provide key experimental endpoints for each of the separate projects that make up this program project, funding is requested for an Assay Core laboratory. The primary purpose of this laboratory will be to establish and perform assays for the measurement of the key regulatory peptides necessary for the completion of the three projects. Routine assays for the core will include insulin, leptin, glucose, corticosterone, gastrin releasing peptide, and cholecystokinin. This core will also be responsible for adapting commercially available kits necessary to measure other hormones and peptides, labeling peptides for use as tracers, processing tissues for assay of peptides and development of new techniques and assays. This core will be utilized extensively by all 3 of the individual projects.

Agency
National Institute of Health (NIH)
Institute
National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK)
Type
Research Program Projects (P01)
Project #
5P01DK056863-10
Application #
8073135
Study Section
Special Emphasis Panel (ZDK1)
Project Start
Project End
2012-10-31
Budget Start
2010-05-01
Budget End
2012-10-31
Support Year
10
Fiscal Year
2010
Total Cost
$188,963
Indirect Cost
Name
University of Cincinnati
Department
Type
DUNS #
041064767
City
Cincinnati
State
OH
Country
United States
Zip Code
45221
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