application) This core will measure tissue, cellular, and mitochondrial levels of ceramide, sphingosine, sphingosine-1-phosphate (S-1-P), diacylglycerol (DAG), and other lipids involved in the regulation of apoptosis. The core will concentrate mostly on the quantitative determination of total ceramide and DAG via DAG-kinase phosphorylation. In selected situations, LC-MS spectroscopic method will be used to obtain both qualitative and quantitative determination of the molecular species of ceramides present in specimens. Determination of the cellular levels of D-erythro-sphingosine and D-erythro-dihydrosphingosine will be based on the BPLC technique developed for their fluorescent analogs. A Synthetic Unit will provide homogenous sphingolipid standards corresponding to the naturally occurring lipids. This core will also synthesize radiolabeled analogs, which will allow the study of sphingolipid metabolic pathways. Key molecules to be synthesized include: stereoisomers of sphingosine, dihydrosphingosine, ceramides, dihydroceramide, sphingosine-1-phosphate and their modified analogs. Also, the core will provide inhibitors of sphingolipid metabolic pathways (e.g., D-e-MAPP).

Agency
National Institute of Health (NIH)
Institute
National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK)
Type
Research Program Projects (P01)
Project #
1P01DK059340-01
Application #
6357656
Study Section
Special Emphasis Panel (ZDK1)
Project Start
2000-12-01
Project End
2005-11-30
Budget Start
Budget End
Support Year
1
Fiscal Year
2001
Total Cost
Indirect Cost
Name
University of North Carolina Chapel Hill
Department
Type
DUNS #
078861598
City
Chapel Hill
State
NC
Country
United States
Zip Code
27599
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