_ROVIDED. Core C, the Molecular Biology and Mouse Support Core, will be directed by S. Russ Price and Susan Wall. The core will provide the molecular biology support necessary to complete the overall goals of the program. Specific responsibilities of the Core include: 1) Preparation of plasmid constructs and adenoviruses for ectopic expression of wild-type or mutant proteins. It will also assist the project investigators with stable and transient cell transfections. 2) Preparation of large peptide fragments for antibody production using an automated protein expression system. Commercial synthesis of large peptides on the scale required for antibody production is not feasible. 3) Maintain systems for imaging and quantification of data from protein and DNA studies. 4) The Core will assist the project investigators in the the genotyping of transgenic and knockout mice. The Core will not duplicate the ability of the Emory University Microchemical Facility to generate oligonucleotides and short peptides, however, the Program does hope to negotiate a volume discount to reduce project costs. Similarly, we will make use of the automated sequencing equipment in the Emory University Department of Anatomy and Cell Biology by underwriting the cost of maintaining the equipment. All four projects will utilize the services of Core C.

Agency
National Institute of Health (NIH)
Institute
National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK)
Type
Research Program Projects (P01)
Project #
5P01DK061521-05
Application #
7674565
Study Section
Special Emphasis Panel (ZDK1)
Project Start
Project End
Budget Start
2008-08-01
Budget End
2009-07-31
Support Year
5
Fiscal Year
2008
Total Cost
$75,295
Indirect Cost
Name
Emory University
Department
Type
DUNS #
066469933
City
Atlanta
State
GA
Country
United States
Zip Code
30322
Blount, Mitsi A; Cipriani, Penelope; Redd, Sara K et al. (2015) Activation of protein kinase C? increases phosphorylation of the UT-A1 urea transporter at serine 494 in the inner medullary collecting duct. Am J Physiol Cell Physiol 309:C608-15
Klein, Janet D; Blount, Mitsi A; Sands, Jeff M (2011) Urea transport in the kidney. Compr Physiol 1:699-729
Huang, Haidong; Yang, Yuan; Eaton, Douglas C et al. (2010) The N-terminal 81-aa fragment is critical for UT-A1 urea transporter bioactivity. J Epithel Biol Pharmacol 3:34-39
Zheng, Bin; Ohkawa, Sakae; Li, Haiyan et al. (2010) FOXO3a mediates signaling crosstalk that coordinates ubiquitin and atrogin-1/MAFbx expression during glucocorticoid-induced skeletal muscle atrophy. FASEB J 24:2660-9
Roberts-Wilson, Tiffany K; Reddy, Ramesh N; Bailey, James L et al. (2010) Calcineurin signaling and PGC-1alpha expression are suppressed during muscle atrophy due to diabetes. Biochim Biophys Acta 1803:960-7
Hu, Junping; Du, Jie; Zhang, Liping et al. (2010) XIAP reduces muscle proteolysis induced by CKD. J Am Soc Nephrol 21:1174-83
Klein, Janet D; Blount, Mitsi A; Frohlich, Otto et al. (2010) Phosphorylation of UT-A1 on serine 486 correlates with membrane accumulation and urea transport activity in both rat IMCDs and cultured cells. Am J Physiol Renal Physiol 298:F935-40
Blount, Mitsi A; Sim, Jae H; Zhou, Rong et al. (2010) Expression of transporters involved in urine concentration recovers differently after cessation of lithium treatment. Am J Physiol Renal Physiol 298:F601-8
Wang, Yanhua; Klein, Janet D; Liedtke, Carole M et al. (2010) Protein kinase C regulates urea permeability in the rat inner medullary collecting duct. Am J Physiol Renal Physiol 299:F1401-6
Park, Hae Jeong; Rajbhandari, Ira; Yang, Han Soo et al. (2010) Neuronal expression of sodium/bicarbonate cotransporter NBCn1 (SLC4A7) and its response to chronic metabolic acidosis. Am J Physiol Cell Physiol 298:C1018-28

Showing the most recent 10 out of 45 publications