Abstract

The Advanced Imaging Core is a state-of-the-art Core.
The specific aims are: '1. Provide equipment and expertise to facilitate the use ofthe mulfiphoton microscope,'Ziess 510/META confocal microscope with FRET and FRAP applications and TIRF microscopy for physiologic studies by PPG investigators. 2. Develop advanced imaging techniques for study of epithelia, especially spectral scanning, FRET, FRAP, and TIRF 3. Provide user training for imaging of cellular and fissue models by mulfiphoton and confocal microscopy. 4. Provide on-going support for experimental design, application of advanced techniques, such as META spectral imaging, FRET, FRAP and TIRF microscopy, methods of image analysis, and reviewing experimental results related to Core C equipment. 5. Maintain Confocal and Mulfiphoton Confocal webpage/signup program. 6. Organize and maintain all Core C equipment and usage records. 7. Provide data for Core A charge-back of Core C use by non-PPG members of the Hopkins community

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK)
Type
Research Program Projects (P01)
Project #
5P01DK072084-06
Application #
8326232
Study Section
Special Emphasis Panel (ZDK1)
Project Start
Project End
Budget Start
2011-09-01
Budget End
2012-08-31
Support Year
6
Fiscal Year
2011
Total Cost
$118,062
Indirect Cost

  Institution

Name
Johns Hopkins University
Department
Type
DUNS #
001910777
City
Baltimore
State
MD
Country
United States
Zip Code
21218

  Publications

Singh, Varsha; Yang, Jianbo; Yin, Jianyi et al. (2018) Cholera toxin inhibits SNX27-retromer-mediated delivery of cargo proteins to the plasma membrane. J Cell Sci 131:
Yin, Jianyi; Tse, Chung-Ming; Avula, Leela Rani et al. (2018) Molecular Basis and Differentiation-Associated Alterations of Anion Secretion in Human Duodenal Enteroid Monolayers. Cell Mol Gastroenterol Hepatol 5:591-609
Sarker, Rafiquel; Cha, Boyoung; Kovbasnjuk, Olga et al. (2017) Phosphorylation of NHE3-S719 regulates NHE3 activity through the formation of multiple signaling complexes. Mol Biol Cell 28:1754-1767
Qiang, Xiaoling; Liotta, Anthony S; Shiloach, Joseph et al. (2017) New melanocortin-like peptide of E. coli can suppress inflammation via the mammalian melanocortin-1 receptor (MC1R): possible endocrine-like function for microbes of the gut. NPJ Biofilms Microbiomes 3:31
Cha, Boyoung; Yang, Jianbo; Singh, Varsha et al. (2017) PDZ domain-dependent regulation of NHE3 protein by both internal Class II and C-terminal Class I PDZ-binding motifs. J Biol Chem 292:8279-8290
Yu, Huimin; Hasan, Nesrin M; In, Julie G et al. (2017) The Contributions of Human Mini-Intestines to the Study of Intestinal Physiology and Pathophysiology. Annu Rev Physiol 79:291-312
Foulke-Abel, Jennifer; In, Julie; Yin, Jianyi et al. (2016) Human Enteroids as a Model of Upper Small Intestinal Ion Transport Physiology and Pathophysiology. Gastroenterology 150:638-649.e8
Gupta, Arnab; Schell, Michael J; Bhattacharjee, Ashima et al. (2016) Myosin Vb mediates Cu+ export in polarized hepatocytes. J Cell Sci 129:1179-89
Zachos, Nicholas C; Kovbasnjuk, Olga; Foulke-Abel, Jennifer et al. (2016) Human Enteroids/Colonoids and Intestinal Organoids Functionally Recapitulate Normal Intestinal Physiology and Pathophysiology. J Biol Chem 291:3759-66
Cebotaru, Liudmila; Liu, Qiangni; Yanda, Murali K et al. (2016) Inhibition of histone deacetylase 6 activity reduces cyst growth in polycystic kidney disease. Kidney Int 90:90-9

Showing the most recent 10 out of 67 publications