This is an interdisciplinary proposal involving 6 departments (biochemistry, botany, crop science, entomology, genetics, and statistics) which is primarily devoted toward the determination of cellular and subcellular events associated with pesticide toxicity. A major effort is directed to elucidation of metabolic pathways involving oxidative and transferase enzymes. These studies include enzyme purification, enzyme reconstitution, and structure-activity relationships. Also included will be cooperative studies (Duke Univ.) involving the neuropathological action of EPN isomers. A closely related project involves studies on dermal and oral absorption of pesticides and the role of blood proteins in their further distribution. Purification of cholinesterases from a number of animal sources will continue, and a study of the biochemical differences among these enzymes is underway. In studies on the mode of action of herbicides, attention is directed to the manner whereby membrane fluidity and permeabilty is altered. Ultrastructural examination is used for confirmation. Genetic studies using Habrabrocon and Artemia as model systems are undertaken to examine possible adverse effects of toxicants on reproduction.

Agency
National Institute of Health (NIH)
Institute
National Institute of Environmental Health Sciences (NIEHS)
Type
Research Program Projects (P01)
Project #
5P01ES000044-25
Application #
3095757
Study Section
Environmental Health Sciences Review Committee (EHS)
Project Start
1976-12-01
Project End
1993-02-28
Budget Start
1990-03-01
Budget End
1991-02-28
Support Year
25
Fiscal Year
1990
Total Cost
Indirect Cost
Name
North Carolina State University Raleigh
Department
Type
Schools of Earth Sciences/Natur
DUNS #
City
Raleigh
State
NC
Country
United States
Zip Code
27695
Bain, L J; McLachlan, J B; LeBlanc, G A (1997) Structure-activity relationships for xenobiotic transport substrates and inhibitory ligands of P-glycoprotein. Environ Health Perspect 105:812-8
LeBlanc, G A; Bain, L J; Wilson, V S (1997) Pesticides: multiple mechanisms of demasculinization. Mol Cell Endocrinol 126:1-5