Core - Human Cell and Tissue Core Facility

Utell, Mark J.

Abstract

Central to the proposed studies is the use of human lung tissue and cells, especially bronchial epithelial cells from which most lung tumors arise. The role of the Human Cell and Tissue Facility is to collect, maintain, and dispense human tissues and cells for studies in the proposed projects. The Core Facility Director will interact with the Dr. Richard Feins, a thoracic surgeon, to collect entire human lungs from organ donors in cases where the lungs will not be used for transplantation. Pulmonary tissues will also be obtained from thoracic surgical specimens. These tissues will be dissected and epithelial cells recovered as required for specific projects. Human epithelial cells will be collected by fiberoptic bronchoscopy from smoking and non-smoking volunteers. A Core Human Cell and Tissue Facility enables us to meet the needs of project investigators for tissues and cells that are maintained under standardized conditions essential for the types of multidisciplinary studies outlined in this proposal. The Facility will supply Projects 2-4 with human lung tissue and cells.

Funding Agency

• Agency: National Institute of Health (NIH)
• Institute: National Institute of Environmental Health Sciences (NIEHS)
• Type: Research Program Projects (P01)
• Project #: 5P01ES007168-02
• Application #: 3733968
• Support Year: 2
• Fiscal Year: 1995

Institution

• Name: Massachusetts Institute of Technology
• City: Cambridge
• State: MA
• Country: United States
• Zip Code: 02139

Publications

Zheng, Weiming; Khrapko, Konstantin; Coller, Hilary A et al. (2006) Origins of human mitochondrial point mutations as DNA polymerase gamma-mediated errors. Mutat Res 599:11-20

Coller, Hilary A; Khrapko, Konstantin; Herrero-Jimenez, Pablo et al. (2005) Clustering of mutant mitochondrial DNA copies suggests stem cells are common in human bronchial epithelium. Mutat Res 578:256-71

Pedersen, Daniel U; Durant, John L; Taghizadeh, Koli et al. (2005) Human cell mutagens in respirable airborne particles from the northeastern United States. 2. Quantification of mutagens and other organic compounds. Environ Sci Technol 39:9547-60

Pedersen, Daniel U; Durant, John L; Penman, Bruce W et al. (2004) Human-cell mutagens in respirable airborne particles in the northeastern United States. 1. Mutagenicity of fractionated samples. Environ Sci Technol 38:682-9

Tomita-Mitchell, Aoy; Ling, Losee Lucy; Glover, Curtis L et al. (2003) The mutational spectrum of the HPRT gene from human T cells in vivo shares a significant concordant set of hot spots with MNNG-treated human cells. Cancer Res 63:5793-8

Luo, Wen; Gurjuar, Rajan; Ozbal, Can et al. (2003) Quantitative detection of benzo[alpha]pyrene diolepoxide-DNA adducts by cryogenic laser induced fluorescence. Chem Res Toxicol 16:74-80

Kim, Andrea S; Thilly, William G (2003) Ligation of high-melting-temperature 'clamp' sequence extends the scanning range of rare point-mutational analysis by constant denaturant capillary electrophoresis (CDCE) to most of the human genome. Nucleic Acids Res 31:e97

Muniappan, Brindha P; Thilly, William G (2002) The DNA polymerase beta replication error spectrum in the adenomatous polyposis coli gene contains human colon tumor mutational hotspots. Cancer Res 62:3271-5

Kim, Andrea S; Holmquist, Gerald P; Thilly, William G (2002) High-efficiency DNA ligation for clamp attachment without polymerase chain reaction. Anal Biochem 310:179-85

Zheng, Weiming; Marcelino, Luisa A; Thilly, William G (2002) Scanning low-frequency point mutants in the mitochondrial genome using constant denaturant capillary electrophoresis. Methods Mol Biol 197:93-106