Abstract

The Function of the Toxin Probes and Assays Core is to provide analytical support to each of the four Research Projects (RP1-4) and to the other Facility Core (FC2). Support will be in the form of (1) toxin standards, (2) toxin antibodies, synaptosomes and pulmonary receptors to be used as reagents for specific binding assays, (3) new toxin-related reagents that may include derivative, natural, or synthetic brevetoxin antagonists, new brevetoxins, mixtures mimicking natural bloom samples, and radiolabeled products, (4) spectroscopy (FTIR, 1-D &2-D NMR, LC-MS and HR-MS) as structural chemistry support for materials isolated from aerosols, water, and mammalian tissues, (5) HPLC separation assistance, both quantitation and methods development, (6) detection expertise and assays for enzyme-linked immunoassay (ELISA) in the laboratory and in the field as well as ELISA kits provided to RPs in support of their laboratory activities requiring range-finding or very rapid turnaround, (7) imtnunocytochemical analyses of tissue and cell samples, and (8) isolation and cultivation of Karenia species present in active red tides to determine the toxigenic origin of brevetoxins.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Environmental Health Sciences (NIEHS)
Type
Research Program Projects (P01)
Project #
5P01ES010594-09
Application #
7879346
Study Section
Special Emphasis Panel (ZES1)
Project Start
Project End
Budget Start
2009-07-01
Budget End
2010-06-30
Support Year
9
Fiscal Year
2009
Total Cost
$280,354
Indirect Cost

  Institution

Name
University of North Carolina Wilmington
Department
Type
DUNS #
040036584
City
Wilmington
State
NC
Country
United States
Zip Code
28403

  Publications

McCall, Jennifer R; Goodman, Allan J; Jacocks, Henry M et al. (2014) Development of a fluorescence assay for the characterization of brevenal binding to rat brain synaptosomes. J Nat Prod 77:2014-20
McCall, Jennifer R; Jacocks, Henry M; Niven, Susan C et al. (2014) Development and utilization of a fluorescence-based receptor-binding assay for the site 5 voltage-sensitive sodium channel ligands brevetoxin and ciguatoxin. J AOAC Int 97:307-15
Goodman, Allan; McCall, Jennifer R; Jacocks, Henry M et al. (2014) Structure activity relationship of brevenal hydrazide derivatives. Mar Drugs 12:1839-58
Gold, Elena P; Jacocks, Henry M; Bourdelais, Andrea J et al. (2013) Brevenal, a brevetoxin antagonist from Karenia brevis, binds to a previously unreported site on mammalian sodium channels. Harmful Algae 26:12-19
McCall, Jennifer R; Jacocks, Henry M; Baden, Daniel G et al. (2012) Development of a competitive fluorescence-based synaptosome binding assay for brevetoxins. Harmful Algae 19:85-91
Zhou, Yue; Sun, Jaijie; Cheng, Yung-Sung (2011) Comparison of deposition in the USP and physical mouth-throat models with solid and liquid particles. J Aerosol Med Pulm Drug Deliv 24:277-84
Bean, Judy A; Fleming, Lora E; Kirkpatrick, Barbara et al. (2011) Florida Red Tide Toxins (Brevetoxins) and Longitudinal Respiratory Effects in Asthmatics. Harmful Algae 10:744-748
Benson, Janet M; Wolf, Molly L; Kajon, Adriana et al. (2011) Brevetoxin inhalation alters the pulmonary response to influenza A in the male F344 rat. J Toxicol Environ Health A 74:313-24
Murrell, Rachel N; Gibson, James E (2011) Brevetoxin 2 alters expression of apoptotic, DNA damage, and cytokine genes in Jurkat cells. Hum Exp Toxicol 30:182-91
Fleming, Lora E; Kirkpatrick, Barbara; Backer, Lorraine C et al. (2011) Review of Florida Red Tide and Human Health Effects. Harmful Algae 10:224-233

Showing the most recent 10 out of 85 publications