1. Antisera raised in mice against purified nuclear and cytoplasmic poly(A)-binding proteins have been used to isolate genes for these proteins from yeast. DNA sequence determination and mutational analysis of the genes are in progess. 2. Preliminary studies have demonstrated the capacity of synthetic peptides to serve as signal sequences for the transport of proteins by nuclear membranes. We propose to exploit this finding to isolate the nuclear signal receptor, determine its location in the nuclear envelope, isolate the gene for the receptor, and reconstitute the transport process in vitro.

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Research Program Projects (P01)
Project #
5P01GM030387-04
Application #
3096123
Study Section
(SSS)
Project Start
1982-07-01
Project End
1986-07-31
Budget Start
1985-07-01
Budget End
1986-07-31
Support Year
4
Fiscal Year
1985
Total Cost
Indirect Cost
Name
Stanford University
Department
Type
Schools of Medicine
DUNS #
800771545
City
Stanford
State
CA
Country
United States
Zip Code
94305
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