Abstract

The Mutagenesis Core, under the supervision of Dr. S. John Mihic, Ph.D., will provide central resources to members of the Program Project. It will be the responsibility of the Mutagenesis Core to produce the glycine, GABAA and glutamate receptor subunit mutants required by the Harris Project for their expression studies. Initially these will be the specific mutants detailed in the Harris proposal. Later, as the modeling studies of Trudell and the structural studies of Overduin, progress, the Core will also make the mutations they require, to test specific hypotheses generated by their work. Beyond our use of traditional and established techniques of mutagenesis, we have developed and will use novel techniques allowing for the concurrent creation of multiple mutants of defined amino acids. The inclusion of a Mutagenesis Core in the Program Project will allow for a centralized facility to produce all of the mutants requested by the Harris Trudell and Overduin Projects in a timely, efficient and cost-effective manner.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Research Program Projects (P01)
Project #
5P01GM047818-07
Application #
6344903
Study Section
Special Emphasis Panel (ZGM1)
Project Start
2000-08-01
Project End
2001-07-31
Budget Start
Budget End
Support Year
7
Fiscal Year
2000
Total Cost
$131,668
Indirect Cost

  Institution

Name
University of California San Francisco
Department
Type
DUNS #
073133571
City
San Francisco
State
CA
Country
United States
Zip Code
94143

  Publications

Cai, Xiaoyun; Huang, Huizhen; Kuzirian, Marissa S et al. (2016) Generation of a KOR-Cre knockin mouse strain to study cells involved in kappa opioid signaling. Genesis 54:29-37
Zarnowska, E D; Rodgers, F C; Oh, I et al. (2015) Etomidate blocks LTP and impairs learning but does not enhance tonic inhibition in mice carrying the N265M point mutation in the beta3 subunit of the GABA(A) receptor. Neuropharmacology 93:171-178
Burkat, Paul M; Lor, Chong; Perouansky, Misha et al. (2014) Enhancement of ?5-containing ?-aminobutyric acid type A receptors by the nonimmobilizer 1,2-dichlorohexafluorocyclobutane (F6) is abolished by the ?3(N265M) mutation. Anesth Analg 119:1277-84
Iyer, Sangeetha V; Chandra, Dave; Homanics, Gregg E (2014) GABAA-R ?4 subunits are required for the low dose locomotor stimulatory effect of alphaxalone, but not for several other behavioral responses to alphaxalone, etomidate or propofol. Neurochem Res 39:1048-56
Blednov, Yuri A; Benavidez, Jill M; Homanics, Gregg E et al. (2012) Behavioral characterization of knockin mice with mutations M287L and Q266I in the glycine receptor ?1 subunit. J Pharmacol Exp Ther 340:317-29
Borghese, Cecilia M; Blednov, Yuri A; Quan, Yu et al. (2012) Characterization of two mutations, M287L and Q266I, in the ?1 glycine receptor subunit that modify sensitivity to alcohols. J Pharmacol Exp Ther 340:304-16
Pearce, R A; Duscher, P; Van Dyke, K et al. (2012) Isoflurane impairs odour discrimination learning in rats: differential effects on short- and long-term memory. Br J Anaesth 108:630-7
Werner, D F; Swihart, A; Rau, V et al. (2011) Inhaled anesthetic responses of recombinant receptors and knockin mice harboring ?2(S270H/L277A) GABA(A) receptor subunits that are resistant to isoflurane. J Pharmacol Exp Ther 336:134-44
Chang, Ki-Young; Park, Young-Gyun; Park, Hye-Yeon et al. (2011) Lack of CaV3.1 channels causes severe motor coordination defects and an age-dependent cerebellar atrophy in a genetic model of essential tremor. Biochem Biophys Res Commun 410:19-23
Harris, R A; Osterndorff-Kahanek, E; Ponomarev, I et al. (2011) Testing the silence of mutations: Transcriptomic and behavioral studies of GABA(A) receptor ýý1 and ýý2 subunit knock-in mice. Neurosci Lett 488:31-5

Showing the most recent 10 out of 167 publications