The extraction of high-resolution data from helically symmetric structures and the interpretation of molecular maps of helical structures in terms of docked atomic models are frontiers in structural cell biology. We propose to develop algorithms to extract higher resolution data from images of helically symmetric structures, to extend our capabilities for docking atomic models of components into molecular maps of complexes of these complexes of these components, to produce a complete set of programs that incorporates state-of-the-art algorithms into a user-friendly package that will be distributed to the EM community, and to produce a clonable, gold-binding tag for electron microscopy. The state-of-the-art developments will be applied (in separately funded studies"""""""") to the bacterial flagellae and to the actin cytoskeleton. In particular the high resolution methods will be used to extend the resolution of the hook and filament from 10A to 4 resolution and of actyomyosin from 20 to 10 A. The docking procedures will be applied to 2D bundles or rafts of actin and actin bundling proteins such as fimbrin, villin and aldolase.
The aim i s to characterize the polymorphism and to derive docked atomic models for the actin bundles.
| Close, William; Neumann, Matthias; Schmidt, Andreas et al. (2018) Physical basis of amyloid fibril polymorphism. Nat Commun 9:699 |
| Loveland, Anna B; Demo, Gabriel; Grigorieff, Nikolaus et al. (2017) Ensemble cryo-EM elucidates the mechanism of translation fidelity. Nature 546:113-117 |
| Liu, Yuhang; Pan, Junhua; Jenni, Simon et al. (2017) CryoEM Structure of an Influenza Virus Receptor-Binding Site Antibody-Antigen Interface. J Mol Biol 429:1829-1839 |
| Abeyrathne, Priyanka D; Koh, Cha San; Grant, Timothy et al. (2016) Ensemble cryo-EM uncovers inchworm-like translocation of a viral IRES through the ribosome. Elife 5: |
| Schmidt, Andreas; Annamalai, Karthikeyan; Schmidt, Matthias et al. (2016) Cryo-EM reveals the steric zipper structure of a light chain-derived amyloid fibril. Proc Natl Acad Sci U S A 113:6200-5 |
| Chou, Hui-Ting; Dukovski, Danijela; Chambers, Melissa G et al. (2016) CATCHR, HOPS and CORVET tethering complexes share a similar architecture. Nat Struct Mol Biol 23:761-3 |
| Dimitrova, Yoana N; Jenni, Simon; Valverde, Roberto et al. (2016) Structure of the MIND Complex Defines a Regulatory Focus for Yeast Kinetochore Assembly. Cell 167:1014-1027.e12 |
| van der Feltz, Clarisse; Pomeranz Krummel, Daniel (2016) Purification of Native Complexes for Structural Study Using a Tandem Affinity Tag Method. J Vis Exp : |
| Loveland, Anna B; Bah, Eugene; Madireddy, Rohini et al. (2016) Ribosome•RelA structures reveal the mechanism of stringent response activation. Elife 5: |
| Baytshtok, Vladimir; Fei, Xue; Grant, Robert A et al. (2016) A Structurally Dynamic Region of the HslU Intermediate Domain Controls Protein Degradation and ATP Hydrolysis. Structure 24:1766-1777 |
Showing the most recent 10 out of 152 publications
