Abstract

The Biochemical and Molecular Biology Core Laboratory of this Program Project will provide ahighly specialized team of professional staff, capable of coordinating a variety of assays in a well-equipped facility.The Core Laboratory will provide Projects I, II and III with the following services:I. Uniform monitoring of sample parameters levels of anesthetic, gas tension, and pH;II. Supply isolated cardiac mitochondria, cardiac myocytes from rat and cultured cells and cellstransfected with relevant recombinant cDNAs;IlI. Biochemical evaluation of mitochondrial energetics, protein kinase C, mitogen-activated proteinkinase and protein tyrosine kinase activity; andIV. Service for confocal microscopy including mitochondrial flavoprotein fluorescence as well asimmunofluorescence.Unlimited access to analytical expertise, highly specialized techniques and equipment in a centralfacility is absolutely essential to each of the proposed projects. Justification for this Core Laboratory is provided in a listing of the protocols and the number of experiments required for each project. In addition, access is available to a Free Radical Core Facility at the Medical College of Wisconsin.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Research Program Projects (P01)
Project #
1P01GM066730-01
Application #
6584878
Study Section
Special Emphasis Panel (ZGM1-PS-0 (01))
Project Start
2002-12-01
Project End
2007-11-30
Budget Start
2002-12-01
Budget End
2004-04-30
Support Year
1
Fiscal Year
2003
Total Cost
$236,308
Indirect Cost

  Institution

Name
Medical College of Wisconsin
Department
Type
DUNS #
937639060
City
Milwaukee
State
WI
Country
United States
Zip Code
53226

  Publications

Liu, Yong; Usa, Kristie; Wang, Feng et al. (2018) MicroRNA-214-3p in the Kidney Contributes to the Development of Hypertension. J Am Soc Nephrol 29:2518-2528
Zhang, Xiao; Dash, Ranjan K; Jacobs, Elizabeth R et al. (2018) Integrated computational model of the bioenergetics of isolated lung mitochondria. PLoS One 13:e0197921
Ghanian, Zahra; Konduri, Girija Ganesh; Audi, Said Halim et al. (2018) Quantitative optical measurement of mitochondrial superoxide dynamics in pulmonary artery endothelial cells. J Innov Opt Health Sci 11:
Liang, Mingyu (2018) Epigenetic Mechanisms and Hypertension. Hypertension 72:1244-1254
Pant, Tarun; Dhanasekaran, Anuradha; Fang, Juan et al. (2018) Current status and strategies of long noncoding RNA research for diabetic cardiomyopathy. BMC Cardiovasc Disord 18:197
Ge, Zhi-Dong; Li, Yingchuan; Qiao, Shigang et al. (2018) Failure of Isoflurane Cardiac Preconditioning in Obese Type 2 Diabetic Mice Involves Aberrant Regulation of MicroRNA-21, Endothelial Nitric-oxide Synthase, and Mitochondrial Complex I. Anesthesiology 128:117-129
Williams, Anna Marie; Liu, Yong; Regner, Kevin R et al. (2018) Artificial intelligence, physiological genomics, and precision medicine. Physiol Genomics 50:237-243
Liu, Pengyuan; Liu, Yong; Liu, Han et al. (2018) Role of DNA De Novo (De)Methylation in the Kidney in Salt-Induced Hypertension. Hypertension 72:1160-1171
Chuppa, Sandra; Liang, Mingyu; Liu, Pengyuan et al. (2018) MicroRNA-21 regulates peroxisome proliferator-activated receptor alpha, a molecular mechanism of cardiac pathology in Cardiorenal Syndrome Type 4. Kidney Int 93:375-389
Korman, Ben; Dash, Ranjan K; Peyton, Philip J (2018) Can Mathematical Modeling Explain the Measured Magnitude of the Second Gas Effect? Anesthesiology 128:1075-1083

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