Abstract

The Analytical Resource Core will provide the researchers affiliated with the Program Project access to high performance analytical instrumentation, mass spectrometry (MS), and nuclear magnetic resonance spectroscopy (NMR), through training, experimental design and routine sample analysis. Coupled to these Instruments are nanocapillary chromatographs that allow separation and analysis of microscale experiments and culture extracts. The ability to analyze small amounts of material is crucial in the effort to employ MS and NMR In medium- and high-throughput screening campaigns for novel phosphonates. The Analytical Resource Core will also develop new methods and analyses that will accelerate the discovery and reengineering portions of the Research Projects.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Research Program Projects (P01)
Project #
2P01GM077596-06
Application #
8329256
Study Section
Special Emphasis Panel (ZRG1-BCMB-U (40))
Project Start
2007-04-10
Project End
2017-03-31
Budget Start
2012-04-10
Budget End
2013-03-31
Support Year
6
Fiscal Year
2012
Total Cost
$234,126
Indirect Cost
$77,515

  Institution

Name
University of Illinois Urbana-Champaign
Department
Type
DUNS #
041544081
City
Champaign
State
IL
Country
United States
Zip Code
61820

  Publications

Parkinson, Elizabeth I; Tryon, James H; Goering, Anthony W et al. (2018) Discovery of the Tyrobetaine Natural Products and Their Biosynthetic Gene Cluster via Metabologenomics. ACS Chem Biol 13:1029-1037
Ulrich, Emily C; Bougioukou, Despina J; van der Donk, Wilfred A (2018) Investigation of Amide Bond Formation during Dehydrophos Biosynthesis. ACS Chem Biol 13:537-541
Wang, Bin; Guo, Fang; Dong, Shi-Hui et al. (2018) Activation of silent biosynthetic gene clusters using transcription factor decoys. Nat Chem Biol :
Goettge, Michelle N; Cioni, Joel P; Ju, Kou-San et al. (2018) PcxL and HpxL are flavin-dependent, oxime-forming N-oxidases in phosphonocystoximic acid biosynthesis in Streptomyces. J Biol Chem 293:6859-6868
Sun, H; Zhao, H; Ang, E L (2018) A coupled chlorinase-fluorinase system with a high efficiency of trans-halogenation and a shared substrate tolerance. Chem Commun (Camb) 54:9458-9461
McLaughlin, Martin I; van der Donk, Wilfred A (2018) Stereospecific Radical-Mediated B12-Dependent Methyl Transfer by the Fosfomycin Biosynthesis Enzyme Fom3. Biochemistry 57:4967-4971
Wang, Yajie; Ren, Hengqian; Zhao, Huimin (2018) Expanding the boundary of biocatalysis: design and optimization of in vitro tandem catalytic reactions for biochemical production. Crit Rev Biochem Mol Biol 53:115-129
Born, David A; Ulrich, Emily C; Ju, Kou-San et al. (2017) Structural basis for methylphosphonate biosynthesis. Science 358:1336-1339
Si, Tong; Li, Bin; Comi, Troy J et al. (2017) Profiling of Microbial Colonies for High-Throughput Engineering of Multistep Enzymatic Reactions via Optically Guided Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry. J Am Chem Soc 139:12466-12473
Peck, Spencer C; Wang, Chen; Dassama, Laura M K et al. (2017) O-H Activation by an Unexpected Ferryl Intermediate during Catalysis by 2-Hydroxyethylphosphonate Dioxygenase. J Am Chem Soc 139:2045-2052

Showing the most recent 10 out of 119 publications