This human Embryonic Stem (hES) Cell Training and Culture Core has two objectives. The first is toprovide centralized hES culture support to all projects; the second is to broadly support hES cell work inour geographic region (Texas/Gulf Coast/South-west) by sponsoring a hES cell training program, tofacilitate adoption of the technology among a larger number of laboratories. For the first objective, we willprovide quality-tested reagents, such as lot-tested media and mouse embryonic fibroblasts (MEF) to allprojects. We will also provide cultures of WA01 (H1)and WA09 (H9)cells to project investigators on aroutine basis, and large-scale cultures of these lines upon request. We will ensure the hES provided toour investigators are high quality through routine testing for sterility, pluripotency, and genetic stability.For the second objective, we will offer a hES Training workshop every other month. This workshop willbe an intensive week-long hands-on training in small groups with highly experienced hES workers.Training will include all basic hES-related technologies, such as MEF preparation and quality testing, hESculture and quality analysis, teratoma formation, and EB differentiation. In addition, an extendedworkshop will be offered annually that will include strategies for genetic modification. This workshop willbe taught in collaboration with the Genetic Modification Core (Core C). By centralizing these efforts ofhES training and culture, we expect to ensure high quality hES culture for our investigators, as well asdissemination of the technology throughout the Texas Medical Center and larger geographic region.
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