Abstract

The purpose of the Molecular Biology Core Facility is to provide the program investigators with the materials, instruments and expertise to carry out research that utilizes molecular biology techniques. The Core Director, Dr. Athena Andreadis will guide this service with the help of an experienced technician. All projects in the grant will use the facility. Dr. Jungalwala will collaborate with Dr. Andreadis to clone and sequence the genes for GlcNAc-transferase and SGGL-1 binding lectins. Dr. McCluer and Dr. Smith will use the facility for the studies on fucosyltransferase genes.Dr. McCaffery will use the facility to isolate and sequence the human gene that encodes the aldehyde dehydrogenase involved in the biosynthesis of retinoic acid. Dr. Andreadis plans to screen for clones and sequence genes interacting with the axonal specific tau transcript and protein. Generating this core facility is a time- efficient and cost-effective way to achieve the goals of the program.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
Eunice Kennedy Shriver National Institute of Child Health & Human Development (NICHD)
Type
Research Program Projects (P01)
Project #
5P01HD005515-30
Application #
6301841
Study Section
Project Start
1999-12-01
Project End
2000-06-30
Budget Start
1998-10-01
Budget End
1999-09-30
Support Year
30
Fiscal Year
2000
Total Cost
$119,073
Indirect Cost

  Institution

Name
Eunice Kennedy Shriver Center Mtl Retardatn
Department
Type
DUNS #
City
Waltham
State
MA
Country
United States
Zip Code
02254

  Publications

Crandall, James E; Goodman, Timothy; McCarthy, Deirdre M et al. (2011) Retinoic acid influences neuronal migration from the ganglionic eminence to the cerebral cortex. J Neurochem 119:723-35
Crandall, James E; McCarthy, Deirdre M; Araki, Kiyomi Y et al. (2007) Dopamine receptor activation modulates GABA neuron migration from the basal forebrain to the cerebral cortex. J Neurosci 27:3813-22
Wang, Junning; Tse, Sze-Wah; Andreadis, Athena (2007) Tau exon 6 is regulated by an intricate interplay of trans factors and cis elements, including multiple branch points. J Neurochem 100:437-45
Araki, Kiyomi Y; Fujimura, Satoshi; MacDonald, Marcy E et al. (2006) Characterization of mouse striatal precursor cell lines expressing functional dopamine receptors. Dev Neurosci 28:518-27
Leroy, Olivier; Wang, Junning; Maurage, Claude-Alain et al. (2006) Brain-specific change in alternative splicing of Tau exon 6 in myotonic dystrophy type 1. Biochim Biophys Acta 1762:460-7
Leroy, Olivier; Dhaenens, Claire-Marie; Schraen-Maschke, Suzanna et al. (2006) ETR-3 represses Tau exons 2/3 inclusion, a splicing event abnormally enhanced in myotonic dystrophy type I. J Neurosci Res 84:852-9
Qu, Qiang; Crandall, James E; Luo, Tuanlian et al. (2006) Defects in tangential neuronal migration of pontine nuclei neurons in the Largemyd mouse are associated with stalled migration in the ventrolateral hindbrain. Eur J Neurosci 23:2877-86
McCaffery, Peter; Zhang, Jinghua; Crandall, James E (2006) Retinoic acid signaling and function in the adult hippocampus. J Neurobiol 66:780-91
Qu, Qiang; Smith, Frances I (2005) Neuronal migration defects in cerebellum of the Largemyd mouse are associated with disruptions in Bergmann glia organization and delayed migration of granule neurons. Cerebellum 4:261-70
McCaffery, Peter; Deutsch, Curtis K (2005) Macrocephaly and the control of brain growth in autistic disorders. Prog Neurobiol 77:38-56

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