Developmental brain abnormalities are a major cause of mental retardation. Appropriate patterning is critical to development of the central nervous system. Compartments of cellular communication coordinated through gap junction channels may be critical for the determination of patterns and their stabilization. Our prior studies have suggested a possible mechanism for forming these compartments and their boundaries - the expression of gap junction subunit proteins (connexins, Cx) that have different permeability and regulatory properties and that modify (or inhibit) the properties of co-expressed connexins. We will evaluate the contributions of five connexins (CX36, CX45, CX56, CX43, and the newly discovered CX33.2) in the development of two regions of the nervous system (hindbrain and spinal cord). Connexin expression will be characterized by in situ hybridization and immunohistochemistry and related to domains of intercellular communication, patterning of gene expression, cell growth, apoptosis, and neuronal migration. The biochemical and functional properties of CX33.2 will be determined. Connexin gene expression and cellular coupling will be modified by gene electroporation, and the developmental consequences determined.
| Domowicz, Miriam; Wadlington, Natasha L; Henry, Judith G et al. (2018) Glial cell responses in a murine multifactorial perinatal brain injury model. Brain Res 1681:52-63 |
| Pusic, Kae M; Pusic, Aya D; Kraig, Richard P (2016) Environmental Enrichment Stimulates Immune Cell Secretion of Exosomes that Promote CNS Myelination and May Regulate Inflammation. Cell Mol Neurobiol 36:313-325 |
| Dawson, Glyn (2016) Quantum dots and potential therapy for Krabbe's disease. J Neurosci Res 94:1293-303 |
| Walters, Ryan; Medintz, Igor L; Delehanty, James B et al. (2015) The Role of Negative Charge in the Delivery of Quantum Dots to Neurons. ASN Neuro 7: |
| Agarwal, Rishabh; Domowicz, Miriam S; Schwartz, Nancy B et al. (2015) Delivery and tracking of quantum dot peptide bioconjugates in an intact developing avian brain. ACS Chem Neurosci 6:494-504 |
| Dawson, Glyn (2015) Measuring brain lipids. Biochim Biophys Acta 1851:1026-39 |
| Pusic, Aya D; Mitchell, Heidi M; Kunkler, Phillip E et al. (2015) Spreading depression transiently disrupts myelin via interferon-gamma signaling. Exp Neurol 264:43-54 |
| Cortes, Mauricio; Cortes, Leslie K; Schwartz, Nancy B (2015) Mapping proteoglycan functions with glycosidases. Methods Mol Biol 1229:443-55 |
| Pusic, Aya D; Kraig, Richard P (2015) Phasic Treatment with Interferon Gamma Stimulates Release of Exosomes that Protect Against Spreading Depression. J Interferon Cytokine Res 35:795-807 |
| Testai, Fernando D; Xu, Hao-Liang; Kilkus, John et al. (2015) Changes in the metabolism of sphingolipids after subarachnoid hemorrhage. J Neurosci Res 93:796-805 |
Showing the most recent 10 out of 158 publications
