Abstract

The Core Facility has provided all of the investigators of the program project with computer-assisted image analysis. The present proposal will allow (1) the basic service component of the facility to be maintained; (2) the addition to the 3D-DIAS an optional confocal front- end and add the necessary software for reconstructing and quantitatively analyzing the dynamic behavior of fluorescent molecular complexes and organelles in three dimensions; (3) the conversion of 3D-DIS to a Macintosh driven workstation; and (4) the development of additional software for the various projects.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
Eunice Kennedy Shriver National Institute of Child Health & Human Development (NICHD)
Type
Research Program Projects (P01)
Project #
2P01HD018577-14
Application #
6240959
Study Section
Project Start
1997-04-01
Project End
1998-03-31
Budget Start
1996-10-01
Budget End
1997-09-30
Support Year
14
Fiscal Year
1997
Total Cost
Indirect Cost

  Institution

Name
University of Iowa
Department
Type
DUNS #
041294109
City
Iowa City
State
IA
Country
United States
Zip Code
52242

  Publications

Soll, David R; Wessels, Deborah; Kuhl, Spencer et al. (2009) How a cell crawls and the role of cortical myosin II. Eukaryot Cell 8:1381-96
Lusche, Daniel F; Wessels, Deborah; Soll, David R (2009) The effects of extracellular calcium on motility, pseudopod and uropod formation, chemotaxis, and the cortical localization of myosin II in Dictyostelium discoideum. Cell Motil Cytoskeleton 66:567-87
Wessels, Deborah; Kuhl, Spencer; Soll, David R (2009) 2D and 3D quantitative analysis of cell motility and cytoskeletal dynamics. Methods Mol Biol 586:315-35
Lin, Jim Jung-Ching; Li, Yan; Eppinga, Robbin D et al. (2009) Chapter 1: roles of caldesmon in cell motility and actin cytoskeleton remodeling. Int Rev Cell Mol Biol 274:1-68
Wessels, Deborah J; Kuhl, Spencer; Soll, David R (2009) Light microscopy to image and quantify cell movement. Methods Mol Biol 571:455-71
Ueda, Atsushi; Wu, Chun-Fang (2009) Effects of social isolation on neuromuscular excitability and aggressive behaviors in Drosophila: altered responses by Hk and gsts1, two mutations implicated in redox regulation. J Neurogenet 23:378-94
Ueda, Atsushi; Wu, Chun-Fang (2009) Role of rut adenylyl cyclase in the ensemble regulation of presynaptic terminal excitability: reduced synaptic strength and precision in a Drosophila memory mutant. J Neurogenet 23:185-99
Ueda, Atsushi; Wu, Chun-Fang (2008) Effects of hyperkinetic, a beta subunit of Shaker voltage-dependent K+ channels, on the oxidation state of presynaptic nerve terminals. J Neurogenet 22:1-13
Lee, J; Ueda, A; Wu, C-F (2008) Pre- and post-synaptic mechanisms of synaptic strength homeostasis revealed by slowpoke and shaker K+ channel mutations in Drosophila. Neuroscience 154:1283-96
Volk, A Paige Davis; Heise, Christine K; Hougen, Jami L et al. (2008) ClC-3 and IClswell are required for normal neutrophil chemotaxis and shape change. J Biol Chem 283:34315-26

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