Abstract

Ectopic collections of neurons in upper layers of neocortex are hypothesized to cause aberrations in information processing in both humans and mouse models of human learning deficits. These ectopic collections are believed to exert their effects by disrupting the appropriate development of neuronal function in both thalamic and neocortical circuits. The experiments described in this proposal represent the first cellular neurophysiological analysis of the effects of neocortical ectopias on neurons in cortex and thalamus. These studies will be conducted by applying whole-cell patch clamp techniques to neurons in living brain slices obtained from genetic mouse models of cortical ectopias, BXSB and NZB mice, and from a surgically induced model of cortical ectopias. The goal of these experiments will be to identify and characterize aberrations in synaptic and cellular physiology associated with neocortical ectopias. This information may lend insight into the cellular mechanisms underlying learning deficits.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
Eunice Kennedy Shriver National Institute of Child Health & Human Development (NICHD)
Type
Research Program Projects (P01)
Project #
5P01HD020806-15
Application #
6450366
Study Section
Project Start
2001-04-01
Project End
2002-08-04
Budget Start
Budget End
Support Year
15
Fiscal Year
2001
Total Cost
Indirect Cost

  Institution

Name
Beth Israel Deaconess Medical Center
Department
Type
DUNS #
076593722
City
Boston
State
MA
Country
United States
Zip Code
02215

  Publications

Chen, Fuyi; Becker, Albert; LoTurco, Joseph (2016) Overview of Transgenic Glioblastoma and Oligoastrocytoma CNS Models and Their Utility in Drug Discovery. Curr Protoc Pharmacol 72:14.37.1-12
Truong, Dongnhu T; Rendall, Amanda R; Rosen, Glenn D et al. (2015) Morphometric changes in subcortical structures of the central auditory pathway in mice with bilateral nodular heterotopia. Behav Brain Res 282:61-9
Che, Alicia; Girgenti, Matthew J; LoTurco, Joseph (2014) The dyslexia-associated gene DCDC2 is required for spike-timing precision in mouse neocortex. Biol Psychiatry 76:387-96
Siddiqi, Faez; Chen, Fuyi; Aron, Abraham W et al. (2014) Fate mapping by piggyBac transposase reveals that neocortical GLAST+ progenitors generate more astrocytes than Nestin+ progenitors in rat neocortex. Cereb Cortex 24:508-20
Fitch, R Holy; Alexander, Michelle L; Threlkeld, Steven W (2013) Early neural disruption and auditory processing outcomes in rodent models: implications for developmental language disability. Front Syst Neurosci 7:58
Truong, Dongnhu T; Bonet, Ashley; Rendall, Amanda R et al. (2013) A behavioral evaluation of sex differences in a mouse model of severe neuronal migration disorder. PLoS One 8:e73144
Tarkar, Aarti; Loges, Niki T; Slagle, Christopher E et al. (2013) DYX1C1 is required for axonemal dynein assembly and ciliary motility. Nat Genet 45:995-1003
Chen, Fuyi; LoTurco, Joseph (2012) A method for stable transgenesis of radial glia lineage in rat neocortex by piggyBac mediated transposition. J Neurosci Methods 207:172-80
Maher, Brady J; LoTurco, Joseph J (2012) Disrupted-in-schizophrenia (DISC1) functions presynaptically at glutamatergic synapses. PLoS One 7:e34053
Threlkeld, Steven W; Hill, Courtney A; Szalkowski, Caitlin E et al. (2012) Effects of test experience and neocortical microgyria on spatial and non-spatial learning in rats. Behav Brain Res 235:130-5

Showing the most recent 10 out of 121 publications