Abstract

This core will provide services that include transformation of lymphocytes, freezing and maintenance of cell lines, preparation of DNA, generation of somatic cell hybrids, and routine cytogenetic assessment of cell lines and lymphocyte cultures, including G-bands and C-bands. Each of these is an essential service required by multiple projects that would have to be duplicated, at considerable cost, in the absence of this core. The core will interact with all four projects and will provide cost savings by centralizing tissue culture activities.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
Eunice Kennedy Shriver National Institute of Child Health & Human Development (NICHD)
Type
Research Program Projects (P01)
Project #
5P01HD032111-05
Application #
6108743
Study Section
Project Start
1999-03-01
Project End
2001-02-28
Budget Start
1998-10-01
Budget End
1999-09-30
Support Year
5
Fiscal Year
1999
Total Cost
Indirect Cost

  Institution

Name
Case Western Reserve University
Department
Type
DUNS #
077758407
City
Cleveland
State
OH
Country
United States
Zip Code
44106

  Publications

Kokotas, Haris; Grigoriadou, Maria; Mikkelsen, Margareta et al. (2009) Investigating the impact of the Down syndrome related common MTHFR 677C>T polymorphism in the Danish population. Dis Markers 27:279-85
Lamb, N E; Sherman, S L; Hassold, T J (2005) Effect of meiotic recombination on the production of aneuploid gametes in humans. Cytogenet Genome Res 111:250-5
Lamb, Neil E; Yu, Kai; Shaffer, John et al. (2005) Association between maternal age and meiotic recombination for trisomy 21. Am J Hum Genet 76:91-9
Schueler, M G; Higgins, A W; Rudd, M K et al. (2001) Genomic and genetic definition of a functional human centromere. Science 294:109-15
Tsuchiya, K; Schueler, M G; Dev, V G (2001) Familial X centromere variant resulting in false-positive prenatal diagnosis of monosomy X by interphase FISH. Prenat Diagn 21:852-5
Brown, A S; Feingold, E; Broman, K W et al. (2000) Genome-wide variation in recombination in female meiosis: a risk factor for non-disjunction of chromosome 21. Hum Mol Genet 9:515-23
Hassold, T; Sherman, S; Hunt, P (2000) Counting cross-overs: characterizing meiotic recombination in mammals. Hum Mol Genet 9:2409-19
Feingold, E; Brown, A S; Sherman, S L (2000) Multipoint estimation of genetic maps for human trisomies with one parent or other partial data. Am J Hum Genet 66:958-68
Hassold, T; Sherman, S (2000) Down syndrome: genetic recombination and the origin of the extra chromosome 21. Clin Genet 57:95-100
Freeman, S B; Yang, Q; Allran, K et al. (2000) Women with a reduced ovarian complement may have an increased risk for a child with Down syndrome. Am J Hum Genet 66:1680-3

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