Abstract

The Animal Core will provide support and funding for the animal related activities for Projects I, II and III. The creation of the Core is a direct response to the increased use of various rodent strains for in vivo studies of the molecular biology of tissue factor. The extensive use of transgenic and knockout animals (generated by other investigators and ourselves) demands careful breeding, documentation and genotyping of offspring. Currently, PAR-1-/-, Factor V Leiden, mTF+/- and transgenic and rescued mice containing the human TF minigene, and its derivatives, are maintained by the Core. The Core will be responsible for the maintenance of these and other lines, distribution of animals to the various projects and generating new lines by breeding and genotyping. The consolidation of all research animal support into a single Core to supervise the conduct of the specific approval protocols of the Program Project has been reviewed and approved by the institutional Animal Research Committee.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Heart, Lung, and Blood Institute (NHLBI)
Type
Research Program Projects (P01)
Project #
5P01HL016411-28
Application #
6464659
Study Section
Project Start
2001-06-01
Project End
2002-05-31
Budget Start
Budget End
Support Year
28
Fiscal Year
2001
Total Cost
Indirect Cost

  Institution

Name
Scripps Research Institute
Department
Type
DUNS #
City
La Jolla
State
CA
Country
United States
Zip Code
92037

  Publications

Kurakula, Kondababu; Koenis, Duco S; Herzik Jr, Mark A et al. (2018) Structural and cellular mechanisms of peptidyl-prolyl isomerase Pin1-mediated enhancement of Tissue Factor gene expression, protein half-life, and pro-coagulant activity. Haematologica 103:1073-1082
Mackman, Nigel; Davis, George E (2011) Blood coagulation and blood vessel development: is tissue factor the missing link? Arterioscler Thromb Vasc Biol 31:2364-6
Lin, Rui; Roychowdhury-Saha, Manami; Black, Chris et al. (2011) Control of RNA processing by a large non-coding RNA over-expressed in carcinomas. FEBS Lett 585:671-6
Niessen, Frank; Furlan-Freguia, Christian; Fernández, José A et al. (2009) Endogenous EPCR/aPC-PAR1 signaling prevents inflammation-induced vascular leakage and lethality. Blood 113:2859-66
Sen, Mehmet; Herzik, Mark; Craft, John W et al. (2009) Spectroscopic Characterization of Successive Phosphorylation of the Tissue Factor Cytoplasmic Region. Open Spectrosc J 3:58-64
Ruf, W; Furlan-Freguia, C; Niessen, F (2009) Vascular and dendritic cell coagulation signaling in sepsis progression. J Thromb Haemost 7 Suppl 1:118-21
van den Berg, Y W; van den Hengel, L G; Myers, H R et al. (2009) Alternatively spliced tissue factor induces angiogenesis through integrin ligation. Proc Natl Acad Sci U S A 106:19497-502
Zhang, Qing; Petersen, Helle H; Ostergaard, Henrik et al. (2009) Molecular dynamics simulations and functional characterization of the interactions of the PAR2 ectodomain with factor VIIa. Proteins 77:559-69
Zhang, Qing; Sanner, Michel; Olson, Arthur J (2009) Shape complementarity of protein-protein complexes at multiple resolutions. Proteins 75:453-67
Potty, Ajish S R; Kourentzi, Katerina; Fang, Han et al. (2009) Biophysical characterization of DNA aptamer interactions with vascular endothelial growth factor. Biopolymers 91:145-56

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