Abstract

The objective of the tissue culture laboratory is to support the investigators of the Program Project who use cells models. A centralized Tissue Culture Core facility has several advantages: 1) it conserves space, eliminates the need for redundant equipment and allows for the efficient acquisition of supplies; 2) it ensures standardization of procedures and cell lines; 3) it facilitates the introduction of new cell models into the program; 4) serves as a training facility. The Tissue Culture Core facility is an integral part of all the projects in the current program.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Heart, Lung, and Blood Institute (NHLBI)
Type
Research Program Projects (P01)
Project #
5P01HL022633-29
Application #
7092171
Study Section
Heart, Lung, and Blood Initial Review Group (HLBP)
Project Start
Project End
Budget Start
2005-07-01
Budget End
2006-06-30
Support Year
29
Fiscal Year
2005
Total Cost
$110,099
Indirect Cost

  Institution

Name
Children's Hospital of Philadelphia
Department
Type
DUNS #
073757627
City
Philadelphia
State
PA
Country
United States
Zip Code
19104

  Publications

Cuchel, Marina; Raper, Anna C; Conlon, Donna M et al. (2017) A novel approach to measuring macrophage-specific reverse cholesterol transport in vivo in humans. J Lipid Res 58:752-762
Nagao, Kohjiro; Hata, Mami; Tanaka, Kento et al. (2014) The roles of C-terminal helices of human apolipoprotein A-I in formation of high-density lipoprotein particles. Biochim Biophys Acta 1841:80-7
Weibel, Ginny L; Drazul-Schrader, Denise; Shivers, Debra K et al. (2014) Importance of evaluating cell cholesterol influx with efflux in determining the impact of human serum on cholesterol metabolism and atherosclerosis. Arterioscler Thromb Vasc Biol 34:17-25
Phillips, Michael C (2014) Molecular mechanisms of cellular cholesterol efflux. J Biol Chem 289:24020-9
Yang, Yanbo; Kuwano, Takashi; Lagor, William R et al. (2014) Lipidomic analyses of female mice lacking hepatic lipase and endothelial lipase indicate selective modulation of plasma lipid species. Lipids 49:505-15
Lagor, William R; Fields, David W; Bauer, Robert C et al. (2014) Genetic manipulation of the ApoF/Stat2 locus supports an important role for type I interferon signaling in atherosclerosis. Atherosclerosis 233:234-41
Sankaranarayanan, Sandhya; de la Llera-Moya, Margarita; Drazul-Schrader, Denise et al. (2013) Serum albumin acts as a shuttle to enhance cholesterol efflux from cells. J Lipid Res 54:671-6
Adachi, Emi; Nakajima, Hiroyuki; Mizuguchi, Chiharu et al. (2013) Dual role of an N-terminal amyloidogenic mutation in apolipoprotein A-I: destabilization of helix bundle and enhancement of fibril formation. J Biol Chem 288:2848-56
Chetty, Palaniappan Sevugan; Nguyen, David; Nickel, Margaret et al. (2013) Comparison of apoA-I helical structure and stability in discoidal and spherical HDL particles by HX and mass spectrometry. J Lipid Res 54:1589-97
Nguyen, David; Nickel, Margaret; Mizuguchi, Chiharu et al. (2013) Interactions of apolipoprotein A-I with high-density lipoprotein particles. Biochemistry 52:1963-72

Showing the most recent 10 out of 336 publications