Abstract

Core B is a tissue culture and MHC typing Core. In this facility, we perform all sample processing that includes the following; isolation of peripheral blood lymphocytes, cryopreservation, thawing and washing of frozen cells and preparation of B cell lines. The Core also perform the following tests: HLA-A, B, C typing by serology on all new samples as well as HLA-DR, DQ serology on a few selected samples. In addition, the Core does DNA typing for HLA Class II alleles using sequence specific oligonucleotide probe hybridization (SSOPH) and DNA typing for HLA-C using sequence-specific primers (PCR-SSP). Other typings, such as complement protein analysis, are also performed on new samples.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Heart, Lung, and Blood Institute (NHLBI)
Type
Research Program Projects (P01)
Project #
5P01HL029583-12
Application #
5213447
Study Section
Project Start
Project End
Budget Start
Budget End
Support Year
12
Fiscal Year
1996
Total Cost
Indirect Cost

  Publications

Feris, Edmond J; Encinales, Liliana; Awad, Carlos et al. (2016) High levels of anti-tuberculin (IgG) antibodies correlate with the blocking of T-cell proliferation in individuals with high exposure to Mycobacterium tuberculosis. Int J Infect Dis 43:21-24
Larsen, Charles E; Alford, Dennis R; Trautwein, Michael R et al. (2014) Dominant sequences of human major histocompatibility complex conserved extended haplotypes from HLA-DQA2 to DAXX. PLoS Genet 10:e1004637
Granados-Montiel, Julio; Zuniga, Joaquin; Azocar, Jose et al. (2011) Interaction between immunoglobulin allotypes and NK receptor genes in diabetes post-hepatitis C virus infection. Immunobiology 216:686-91
Encinales, Liliana; Zuniga, Joaquin; Granados-Montiel, Julio et al. (2010) Humoral immunity in tuberculin skin test anergy and its role in high-risk persons exposed to active tuberculosis. Mol Immunol 47:1066-73
Szilagyi, Agnes; Banlaki, Zsofia; Pozsonyi, Eva et al. (2010) Frequent occurrence of conserved extended haplotypes (CEHs) in two Caucasian populations. Mol Immunol 47:1899-904
Zúñiga, Joaquín; Romero, Viviana; Azocar, José et al. (2009) Protective KIR-HLA interactions for HCV infection in intravenous drug users. Mol Immunol 46:2723-7
Stern, Joel N H; Keskin, Derin B; Romero, Viviana et al. (2009) Molecular signatures distinguishing active from latent tuberculosis in peripheral blood mononuclear cells, after in vitro antigenic stimulation with purified protein derivative of tuberculin (PPD) or Candida: a preliminary report. Immunol Res 45:1-12
Husain, Zaheed; Kelly, M Ann; Eisenbarth, George S et al. (2008) The MHC type 1 diabetes susceptibility gene is centromeric to HLA-DQB1. J Autoimmun 30:266-72
Romero, Viviana; Zuniga, Joaquin; Azocar, Jose et al. (2008) Genetic interactions of KIR and G1M immunoglobulin allotypes differ in obese from non-obese individuals with type 2 diabetes. Mol Immunol 45:3857-62
Romero, Viviana; Azocar, Jose; Zuniga, Joaquin et al. (2008) Interaction of NK inhibitory receptor genes with HLA-C and MHC class II alleles in Hepatitis C virus infection outcome. Mol Immunol 45:2429-36

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