Core B will provide services to the component projects in three areas of expertise. (1) Analytical Chemistry related services: Core B will perform quantitative analysis of lipids, lipoproteins, hormones, enzyme activities, and various plasma metabolites that have relevance to atherosclerosis and lipid metabolism. (2) Molecular Biology related services: Core B will assist in the construction of novel, genetically modified animal models by gene targeting and transgenic mouse techniques. This core will also help in the construction of recombinant adenoviruses. (3) Morphology related services: Core B will provide quantitative and detailed cellular analysis of atherosclerotic lesions in the aortic root and innominate arteries of mice and will provide quantitative analysis of the percent of aorta with atherosclerotic lesions using en face analysis. Core B will also offer immunocytochemistry and immunohistology related services. All component projects are expected to use the services provided by Core B in the next grant cycle.
The core assists the component projects in generating genetically modified animal models and performing various tests on samples collected from animals, tissues, and cells for the study of lipid and lipoprotein metabolism in atherosclerosis. This is highly relevant to public health since atherosclerosis is a major cause for cardiovascular diseases such as coronary heart disease and stroke. Findings from this program project can potentially lead to the development of novel therapeutics for cardiovascular disease.
Showing the most recent 10 out of 791 publications
