Core D: The imaging of airway epithelia is crucial to understanding the function and control of airway surfaceliquid in both health and disease. The Imaging Core will provide the skills and resources necessary to fix,embed, stain and image tissues and cells, or stain and image live tissues for light and electron microscopy.An extensive range of standard microscopy techniques will be made available. Hence, the Core will enablethe localization by immunostaining of CFTR, ENaC, and proteases of interest created by the projects, trackand assess lipids and vesicle trafficking in live tissues, and measure airway surface liquid levels on culturedtissue models. Imaging Core resources will be used by the Core personnel as a service to projectparticipants, and optical equipment and general image analysis users will be trained by the Core personnelin the proper and knowledgeable use of specific equipment. Consultation on tissue preparation, staining,image acquisition and analysis will be provided. Core personnel will assist project investigators improve anddevelop standard and new techniques, trouble shoot problems, and help ensure that results are interpretedmeaningfully.Relevance to public health: The Imaging Core provides high performance microscopy to the researchprojects in terms of acquisition, analysis and interpretation.
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