A. STATEMENT OF OBJECTIVESThe Cell Biology Core is a new facility and will serve all four projects. This core will provideseveral lipoprotein-producing and non-lipoprotein producing cell lines required for studiesdescribed in each project.The lipoprotein producing cells will be used: 1) To study the mechanisms of assembly andsecretion of endogenous apoA-l- and apoB-containing nascent lipoprotein particles; 2) Todetermine the effects of several apoA-l mimetic peptides on the concentration, composition, andproperties of apoA-l- and apoB-containing nascent lipoprotein particles; 3) For transfectionstudies to assess the structure-function relationship of apoB, and potentially apoA-l, and theirrespective particle assembly and secretion; 4) To silence the gene of interest using miRNAtechnique; 5) To establish clonal stable transformants of cells that express C-terminallytruncated forms of human apoB or cells that lack specific gene, i.e, lipid transfer proteins.The non-lipoprotein producing cell lines will be used: 1) For the expression of ABCA1,ABCG1, and ABCG4; 2) To established clonal statble cell lines overexpressing ABCA1,ABCG1, and ABCG4; 3) To evaluate the effects of apoA-l mimetic peptides on LPS-inducedadhesion of THP-1 monocytes to HUVECs; 4) For cholesterol efflux studies.
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