Abstract

The Morphology/Immunology/Biochemistry Section will provide the various projects in the program with the necessary expertise, facilities, and support to perform routine light microscopy, immunofluorescent microscopy and any other routine or specialized morphological, pathological and immunohistochemical support required. The Molecular Biology Section will supply the program with the capabilities to prepare and test the role of specific domains of the connexin proteins on their function and pH sensitivity. Specifically the responsibilities of the Molecular Biology Section of Core C will include the growth and purification of DNA; site-specific mutagenesis of the connexin cDNA's; production of cRNA and checking of the cRNA in vitro translation assays; DNA sequencing of mutagenized connexin and cDNA's preparation and microinjection of frog oocytes; and expression of connexins by transfection into mammalian cells in culture.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Heart, Lung, and Blood Institute (NHLBI)
Type
Research Program Projects (P01)
Project #
5P01HL039707-12
Application #
6459573
Study Section
Project Start
2001-05-01
Project End
2002-04-30
Budget Start
Budget End
Support Year
12
Fiscal Year
2001
Total Cost
$282,265
Indirect Cost

  Institution

Name
Upstate Medical University
Department
Type
DUNS #
058889106
City
Syracuse
State
NY
Country
United States
Zip Code
13210

  Publications

Ponce-Balbuena, Daniela; Guerrero-Serna, Guadalupe; Valdivia, Carmen R et al. (2018) Cardiac Kir2.1 and NaV1.5 Channels Traffic Together to the Sarcolemma to Control Excitability. Circ Res 122:1501-1516
Rodrigo, M; Climent, A M; Liberos, A et al. (2017) Minimal configuration of body surface potential mapping for discrimination of left versus right dominant frequencies during atrial fibrillation. Pacing Clin Electrophysiol 40:940-946
Rodrigo, Miguel; Climent, Andreu M; Liberos, Alejandro et al. (2017) Highest dominant frequency and rotor positions are robust markers of driver location during noninvasive mapping of atrial fibrillation: A computational study. Heart Rhythm 14:1224-1233
Herron, Todd J; Rocha, Andre Monteiro Da; Campbell, Katherine F et al. (2016) Extracellular Matrix-Mediated Maturation of Human Pluripotent Stem Cell-Derived Cardiac Monolayer Structure and Electrophysiological Function. Circ Arrhythm Electrophysiol 9:e003638
Guillem, María S; Climent, Andreu M; Rodrigo, Miguel et al. (2016) Presence and stability of rotors in atrial fibrillation: evidence and therapeutic implications. Cardiovasc Res 109:480-92
Willis, B Cicero; Pandit, Sandeep V; Ponce-Balbuena, Daniela et al. (2016) Constitutive Intracellular Na+ Excess in Purkinje Cells Promotes Arrhythmogenesis at Lower Levels of Stress Than Ventricular Myocytes From Mice With Catecholaminergic Polymorphic Ventricular Tachycardia. Circulation 133:2348-59
Quintanilla, Jorge G; Pérez-Villacastín, Julián; Pérez-Castellano, Nicasio et al. (2016) Mechanistic Approaches to Detect, Target, and Ablate the Drivers of Atrial Fibrillation. Circ Arrhythm Electrophysiol 9:e002481
Takemoto, Yoshio; Ramirez, Rafael J; Yokokawa, Miki et al. (2016) Galectin-3 Regulates Atrial Fibrillation Remodeling and Predicts Catheter Ablation Outcomes. JACC Basic Transl Sci 1:143-154
Filgueiras-Rama, David; Jalife, José (2016) STRUCTURAL AND FUNCTIONAL BASES OF CARDIAC FIBRILLATION. DIFFERENCES AND SIMILARITIES BETWEEN ATRIA AND VENTRICLES. JACC Clin Electrophysiol 2:1-3
Pedrón-Torrecilla, Jorge; Rodrigo, Miguel; Climent, Andreu M et al. (2016) Noninvasive Estimation of Epicardial Dominant High-Frequency Regions During Atrial Fibrillation. J Cardiovasc Electrophysiol 27:435-42

Showing the most recent 10 out of 257 publications