Abstract

The Hybridoma Core Laboratory provides a centralized resource for the Program for all aspects of monoclonal antibody (mAb) production including 1) immunization of animals (Balb/c mice), 2) screening of animal sera for immune responses, 3) performing fusion protocols and all cell culture related to the production, cloning and establishment of stable hybridoma cell lines, 4) performing screening protocols for mAbs using ELISA, Western blot, immunofluorescence microscopy, and flow cytometry, 5) producing and purifying selected mAbs from ascitic fluid, 6) isotyping mAbs, and 7) conjugating selected mAbs for particular experiments (ie. to FITC or biotin).

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Heart, Lung, and Blood Institute (NHLBI)
Type
Research Program Projects (P01)
Project #
5P01HL040387-15
Application #
6573411
Study Section
Project Start
2002-04-01
Project End
2003-03-31
Budget Start
1998-10-01
Budget End
1999-09-30
Support Year
15
Fiscal Year
2002
Total Cost
$197,196
Indirect Cost

  Institution

Name
University of Pennsylvania
Department
Type
DUNS #
042250712
City
Philadelphia
State
PA
Country
United States
Zip Code
19104

  Publications

Capitano, Maegan; Zhao, Liang; Cooper, Scott et al. (2018) Phosphatidylinositol transfer proteins regulate megakaryocyte TGF-?1 secretion and hematopoiesis in mice. Blood 132:1027-1038
Branchford, B R; Stalker, T J; Law, L et al. (2018) The small-molecule MERTK inhibitor UNC2025 decreases platelet activation and prevents thrombosis. J Thromb Haemost 16:352-363
Zhao, Baobing; Mei, Yang; Cao, Lan et al. (2018) Loss of pleckstrin-2 reverts lethality and vascular occlusions in JAK2V617F-positive myeloproliferative neoplasms. J Clin Invest 128:125-140
Khandelwal, Sanjay; Ravi, Joann; Rauova, Lubica et al. (2018) Polyreactive IgM initiates complement activation by PF4/heparin complexes through the classical pathway. Blood 132:2431-2440
Villa, Carlos H; Pan, Daniel C; Johnston, Ian H et al. (2018) Biocompatible coupling of therapeutic fusion proteins to human erythrocytes. Blood Adv 2:165-176
Ma, Peisong; Gupta, Shuchi; Sampietro, Sara et al. (2018) RGS10 shapes the hemostatic response to injury through its differential effects on intracellular signaling by platelet agonists. Blood Adv 2:2145-2155
Gupta, Shuchi; Cherpokova, Deya; Spindler, Markus et al. (2018) GPVI signaling is compromised in newly formed platelets after acute thrombocytopenia in mice. Blood 131:1106-1110
Xie, Zhigang; Hur, Seong Kwon; Zhao, Liang et al. (2018) A Golgi Lipid Signaling Pathway Controls Apical Golgi Distribution and Cell Polarity during Neurogenesis. Dev Cell 44:725-740.e4
Kononova, Olga; Litvinov, Rustem I; Blokhin, Dmitry S et al. (2017) Mechanistic Basis for the Binding of RGD- and AGDV-Peptides to the Platelet Integrin ?IIb?3. Biochemistry 56:1932-1942
Höök, Peter; Litvinov, Rustem I; Kim, Oleg V et al. (2017) Strong Binding of Platelet Integrin ?IIb?3 to Fibrin Clots: Potential Target to Destabilize Thrombi. Sci Rep 7:13001

Showing the most recent 10 out of 290 publications