Abstract

The Instrumentation Core will provide all projects and principle investigators of this Program Project Grant with: a) confocal and conventional light microscopy and image analyses of fixed samples for localization and colocalization experiments, as well as quantification of morphological changes. b) ratiometric fluorescence microscopy of live cells for dynamic measurements of intracellular ion concentration of membrane potential changes. c) microinjection of proteins and nucleic acids to modulate intracellular message or signaling systems. d) injection of proteins into cells by electroporation to effect modulation of intracellular signaling system in biochemical studies. e) routine resistance and potential measurements of epithelial monolayers as quality control. f) quantitative analyses of protein and nucleic aid gels and immuno-blots. The staff of the Core will carry out measurements and provide assistance and counseling in planning of measurements and data analyses.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Heart, Lung, and Blood Institute (NHLBI)
Type
Research Program Projects (P01)
Project #
2P01HL041618-11A1
Application #
6315346
Study Section
Project Start
2000-05-15
Project End
2001-04-30
Budget Start
Budget End
Support Year
11
Fiscal Year
2000
Total Cost
$259,467
Indirect Cost

  Institution

Name
Case Western Reserve University
Department
Type
DUNS #
077758407
City
Cleveland
State
OH
Country
United States
Zip Code
44106

  Publications

Huang, Chunfa; Miller, Richard Tyler (2010) Novel Ca receptor signaling pathways for control of renal ion transport. Curr Opin Nephrol Hypertens 19:106-12
Yu, Changqing; Yang, Zhiwei; Ren, Hongmei et al. (2009) D3 dopamine receptor regulation of ETB receptors in renal proximal tubule cells from WKY and SHRs. Am J Hypertens 22:877-83
Zeng, Chunyu; Asico, Laureano D; Yu, Changqing et al. (2008) Renal D3 dopamine receptor stimulation induces natriuresis by endothelin B receptor interactions. Kidney Int 74:750-9
Zeng, Chunyu; Armando, Ines; Luo, Yingjin et al. (2008) Dysregulation of dopamine-dependent mechanisms as a determinant of hypertension: studies in dopamine receptor knockout mice. Am J Physiol Heart Circ Physiol 294:H551-69
Zeng, Chunyu; Villar, Van Anthony M; Eisner, Gilbert M et al. (2008) G protein-coupled receptor kinase 4: role in blood pressure regulation. Hypertension 51:1449-55
Resnick, Andrew; Hopfer, Ulrich (2008) Mechanical stimulation of primary cilia. Front Biosci 13:1665-80
Huang, Chunfa; Miller, R Tyler (2007) Regulation of renal ion transport by the calcium-sensing receptor: an update. Curr Opin Nephrol Hypertens 16:437-43
Ulmasov, Barbara; Bruno, Jonathan; Woost, Philip G et al. (2007) Tissue and subcellular distribution of CLIC1. BMC Cell Biol 8:8
Tandon, R; Levental, I; Huang, C et al. (2007) HIV infection changes glomerular podocyte cytoskeletal composition and results in distinct cellular mechanical properties. Am J Physiol Renal Physiol 292:F701-10
Huang, Chunfa; Sindic, Aleksandra; Hill, Ceredwyn E et al. (2007) Interaction of the Ca2+-sensing receptor with the inwardly rectifying potassium channels Kir4.1 and Kir4.2 results in inhibition of channel function. Am J Physiol Renal Physiol 292:F1073-81

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