Abstract

The overall goal of Core A is to provide training, tools, technologies and model systems that will enable the projects of the PPG to analyze the structure and function of key adhesive junctions of the cardiac myocyte and to investigate how defects in cardiac cell-cell and cell-extracellular matrix molecular complexes disrupt structural integrity, electrical, mechanical and signaling functions in isolated cardiac cells, tissues and whole hearts. In particular, Core A will provide expertise and techniques to (a) image and analyze myocardial and myocyte structures including intercalated discs, costameres and their components;and (b) characterize cardiac muscle electrical, mechanical and signaling functions in three types of experimental preparation: isolated murine cardiomyocytes;isolated mouse trabeculae and papillary muscles;and isolated perfused adult or neonatal mouse hearts. In addition, Core A will bring expertise in bioengineering and biophysics, biophotonics and microscopy to collaborate with all three projects and the other cores in the design and development of new preparations, techniques and assays for analyzing structure and biophysical function mouse heart cells and tissues.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Heart, Lung, and Blood Institute (NHLBI)
Type
Research Program Projects (P01)
Project #
5P01HL046345-20
Application #
8316263
Study Section
Heart, Lung, and Blood Initial Review Group (HLBP)
Project Start
Project End
2014-06-30
Budget Start
2011-07-01
Budget End
2013-06-30
Support Year
20
Fiscal Year
2011
Total Cost
$310,717
Indirect Cost

  Institution

Name
University of California San Diego
Department
Type
DUNS #
804355790
City
La Jolla
State
CA
Country
United States
Zip Code
92093

  Publications

Dewan, Sukriti; McCabe, Kimberly J; Regnier, Michael et al. (2016) Molecular Effects of cTnC DCM Mutations on Calcium Sensitivity and Myofilament Activation-An Integrated Multiscale Modeling Study. J Phys Chem B 120:8264-75
Peter, Angela K; Bradford, William H; Dalton, Nancy D et al. (2016) Increased Echogenicity and Radiodense Foci on Echocardiogram and MicroCT in Murine Myocarditis. PLoS One 11:e0159971
Sheikh, Farah; Lyon, Robert C; Chen, Ju (2015) Functions of myosin light chain-2 (MYL2) in cardiac muscle and disease. Gene 569:14-20
Israeli-Rosenberg, Sharon; Chen, Chao; Li, Ruixia et al. (2015) Caveolin modulates integrin function and mechanical activation in the cardiomyocyte. FASEB J 29:374-84
Stroud, Matthew J; Banerjee, Indroneal; Veevers, Jennifer et al. (2014) Linker of nucleoskeleton and cytoskeleton complex proteins in cardiac structure, function, and disease. Circ Res 114:538-48
Zemljic-Harpf, Alice E; Godoy, Joseph C; Platoshyn, Oleksandr et al. (2014) Vinculin directly binds zonula occludens-1 and is essential for stabilizing connexin-43-containing gap junctions in cardiac myocytes. J Cell Sci 127:1104-16
Lyon, Robert C; Mezzano, Valeria; Wright, Adam T et al. (2014) Connexin defects underlie arrhythmogenic right ventricular cardiomyopathy in a novel mouse model. Hum Mol Genet 23:1134-50
Pfeiffer, E R; Wright, A T; Edwards, A G et al. (2014) Caveolae in ventricular myocytes are required for stretch-dependent conduction slowing. J Mol Cell Cardiol 76:265-74
Bang, Marie-Louise; Gu, Yusu; Dalton, Nancy D et al. (2014) The muscle ankyrin repeat proteins CARP, Ankrd2, and DARP are not essential for normal cardiac development and function at basal conditions and in response to pressure overload. PLoS One 9:e93638
Israeli-Rosenberg, Sharon; Manso, Ana Maria; Okada, Hideshi et al. (2014) Integrins and integrin-associated proteins in the cardiac myocyte. Circ Res 114:572-586

Showing the most recent 10 out of 144 publications