Abstract

The service of this Core Unit will be preparation of oxygen carriers and of the fluids to be used in vivo. Other services will be purification of recombinant and chemically modified hemoglobins. Services of the Core: 1) Purification of oxygen carriers. 2) Preparation of the fluids to be used in vivo. 3) Purification of mutant hemoglobins natural and obtained by recombinant techniques.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Heart, Lung, and Blood Institute (NHLBI)
Type
Research Program Projects (P01)
Project #
5P01HL048517-04
Application #
5213973
Study Section
Project Start
Project End
Budget Start
Budget End
Support Year
4
Fiscal Year
1996
Total Cost
Indirect Cost

  Publications

Bucci, Enrico; Pucciarelli, Stefania; Angeletti, Mauro (2013) Free energy changes and components implicit in the MWC allosteric model for the cooperative oxygen binding of hemoglobin. Biochemistry 52:4149-56
Koehler, Raymond C; Fronticelli, Clara; Bucci, Enrico (2008) Insensitivity of cerebral oxygen transport to oxygen affinity of hemoglobin-based oxygen carriers. Biochim Biophys Acta 1784:1387-94
Arosio, Daniele; Kwansa, Herman E; Gering, Henry et al. (2002) Static and dynamic light scattering approach to the hydration of hemoglobin and its supertetramers in the presence of osmolites. Biopolymers 63:1-11
Fronticelli, Clara; Bobofchak, Kevin M; Karavitis, Michael et al. (2002) Introduction of a new regulatory mechanism into human hemoglobin. Biophys Chem 98:115-26
Rebel, Annette; Ulatowski, John A; Joung, Karena et al. (2002) Regional cerebral blood flow in cats with cross-linked hemoglobin transfusion during focal cerebral ischemia. Am J Physiol Heart Circ Physiol 282:H832-41
Gattoni, M; Piro, M C; Boffi, A et al. (2001) The heme-globin and dimerization equilibria of recombinant human hemoglobins carrying site-specific beta chains mutations. Arch Biochem Biophys 386:172-8
Fronticelli, C; Arosio, D; Bobofchak, K M et al. (2001) Molecular engineering of a polymer of tetrameric hemoglobins. Proteins 44:212-22
Piro, M C; Militello, V; Leone, M et al. (2001) Heme pocket disorder in myoglobin: reversal by acid-induced soft refolding. Biochemistry 40:11841-50
Kwansa, H E; Young, A D; Arosio, D et al. (2000) Adipyl crosslinked bovine hemoglobins as new models of allosteric systems. Proteins 39:166-9
Matheson, B; Razynska, A; Kwansa, H et al. (2000) Appearance of dissociable and cross-linked hemoglobins in the renal hilar lymph. J Lab Clin Med 135:459-64

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