Abstract

Core C: Tissue Analyses Core C will provide centralized processing, analysis, and quantitation of various tissues and fluids from the individual projects. This facility will primarily use histopathological approaches and biochemical measurements. The histopathological aspects of the Core will build on our extensive experience in analysis and quantitation of mouse atherosclerosis and of human tissue specimens, notably atheromata. Among the biochemical methods routinely employed in our laboratory, analysis of inflammatory mediators such as cytokines by ELISA is routine. We also have facilities for and experience in measuring mouse lipoprotein profiles by fast pressure liquid chromatography (FPLC). We have specialized personnel dedicated to both the histopathological and biochemical aspects of the services of this Core. Dr. Galina K. Sukhova will supervise the histopathological functions of this Core. I will do so for the biochemical functions, and I will provide overall guidance and oversight and will be responsible for smooth conduct of the various functions of the Core, prioritize access to the Core's facilities by individual projects and aid the design and interpretation of experiments regarding his area of expertise to the individual investigators.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Heart, Lung, and Blood Institute (NHLBI)
Type
Research Program Projects (P01)
Project #
5P01HL048743-17
Application #
7840461
Study Section
Heart, Lung, and Blood Initial Review Group (HLBP)
Project Start
Project End
Budget Start
2009-04-01
Budget End
2010-03-31
Support Year
17
Fiscal Year
2009
Total Cost
$176,617
Indirect Cost

  Institution

Name
Brigham and Women's Hospital
Department
Type
DUNS #
030811269
City
Boston
State
MA
Country
United States
Zip Code
02115

  Publications

Steinhorn, Benjamin; Sorrentino, Andrea; Badole, Sachin et al. (2018) Chemogenetic generation of hydrogen peroxide in the heart induces severe cardiac dysfunction. Nat Commun 9:4044
Brown, Jonathan D; Feldman, Zachary B; Doherty, Sean P et al. (2018) BET bromodomain proteins regulate enhancer function during adipogenesis. Proc Natl Acad Sci U S A 115:2144-2149
Samokhin, Andriy O; Stephens, Thomas; Wertheim, Bradley M et al. (2018) NEDD9 targets COL3A1 to promote endothelial fibrosis and pulmonary arterial hypertension. Sci Transl Med 10:
Pang, Paul; Abbott, Molly; Abdi, Malyun et al. (2018) Pre-clinical model of severe glutathione peroxidase-3 deficiency and chronic kidney disease results in coronary artery thrombosis and depressed left ventricular function. Nephrol Dial Transplant 33:923-934
Steinhorn, Benjamin; Sartoretto, Juliano L; Sorrentino, Andrea et al. (2017) Insulin-dependent metabolic and inotropic responses in the heart are modulated by hydrogen peroxide from NADPH-oxidase isoforms NOX2 and NOX4. Free Radic Biol Med 113:16-25
Handy, Diane E; Loscalzo, Joseph (2017) Responses to reductive stress in the cardiovascular system. Free Radic Biol Med 109:114-124
Liu, Cong-Lin; Wang, Yi; Liao, Mengyang et al. (2016) Allergic Lung Inflammation Aggravates Angiotensin II-Induced Abdominal Aortic Aneurysms in Mice. Arterioscler Thromb Vasc Biol 36:69-77
Machlus, Kellie R; Wu, Stephen K; Stumpo, Deborah J et al. (2016) Synthesis and dephosphorylation of MARCKS in the late stages of megakaryocyte maturation drive proplatelet formation. Blood 127:1468-80
Liu, Cong-Lin; Wemmelund, Holger; Wang, Yi et al. (2016) Asthma Associates With Human Abdominal Aortic Aneurysm and Rupture. Arterioscler Thromb Vasc Biol 36:570-8
Garmaroudi, Farshid S; Handy, Diane E; Liu, Yang-Yu et al. (2016) Systems Pharmacology and Rational Polypharmacy: Nitric Oxide-Cyclic GMP Signaling Pathway as an Illustrative Example and Derivation of the General Case. PLoS Comput Biol 12:e1004822

Showing the most recent 10 out of 266 publications