Abstract

The objective of this core facility is to provide the technical and scientific support for the research projects through the isolation of rare or specific cell populations, the amplification of RNA isolated from the respective cell populations and the comprehensive analysis of the genetic program of these cell populations using microarray analysis. Specifically, we have established the following expertise, which will be provided for the support of each of the projects. 1) Analysis of cell populations using FACS analysis; 2) RNA micro-isolation; 3) Linear RNA amplification; 4) Microarray analysis; and 5) Bioinformatics of microarray data for the initial analysis and integration of results with previous studies. This is an extension of an ongoing collaboation between Dr. Garry and the project leaders.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Heart, Lung, and Blood Institute (NHLBI)
Type
Research Program Projects (P01)
Project #
7P01HL049953-13
Application #
7071811
Study Section
Project Start
Project End
Budget Start
2005-06-01
Budget End
2006-05-31
Support Year
13
Fiscal Year
2005
Total Cost
$300,415
Indirect Cost

  Institution

Name
Texas A&M University
Department
Type
DUNS #
835607441
City
College Station
State
TX
Country
United States
Zip Code
77845

  Publications

Liu, Yu; Kaneda, Ruri; Leja, Thomas W et al. (2014) Hhex and Cer1 mediate the Sox17 pathway for cardiac mesoderm formation in embryonic stem cells. Stem Cells 32:1515-26
Zeve, Daniel; Seo, Jin; Suh, Jae Myoung et al. (2012) Wnt signaling activation in adipose progenitors promotes insulin-independent muscle glucose uptake. Cell Metab 15:492-504
Verzi, Michael P; Stanfel, Monique N; Moses, Kelvin A et al. (2009) Role of the homeodomain transcription factor Bapx1 in mouse distal stomach development. Gastroenterology 136:1701-10
Niu, Zhivy; Li, Ankang; Zhang, Shu X et al. (2007) Serum response factor micromanaging cardiogenesis. Curr Opin Cell Biol 19:618-27
Shah, Viraj R; Koster, Maranke I; Roop, Dennis R et al. (2007) Double-inducible gene activation system for caspase 3 and 9 in epidermis. Genesis 45:194-9
Chang, Jiang; Xie, Min; Shah, Viraj R et al. (2006) Activation of Rho-associated coiled-coil protein kinase 1 (ROCK-1) by caspase-3 cleavage plays an essential role in cardiac myocyte apoptosis. Proc Natl Acad Sci U S A 103:14495-500
Zhang, Ying-Min; Bo, Jacqueline; Taffet, George E et al. (2006) Targeted deletion of ROCK1 protects the heart against pressure overload by inhibiting reactive fibrosis. FASEB J 20:916-25
Ilagan, Roger; Abu-Issa, Radwan; Brown, Doris et al. (2006) Fgf8 is required for anterior heart field development. Development 133:2435-45
Zhang, Shu Xing; Garcia-Gras, Eduardo; Wycuff, Diane R et al. (2005) Identification of direct serum-response factor gene targets during Me2SO-induced P19 cardiac cell differentiation. J Biol Chem 280:19115-26
Vlahopoulos, Spiros; Zimmer, Warren E; Jenster, Guido et al. (2005) Recruitment of the androgen receptor via serum response factor facilitates expression of a myogenic gene. J Biol Chem 280:7786-92

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