Abstract

The component studies in this Program Project on the genetics of early cardiac development share a technical and biological focus on the mouse as the organism studied. Among vertebrates, only the mouse permits so robust a range of genetic manipulations. The projects involve creating mutations in the germ line and somatic tissues of mice by performing homologous recombination in ES cells, or Cre/IoxP recombination in vivo. Other needs include the creation of mice harboring a bacterial artificial chromosome (BAC), conventional transgene, inducible transgene, or engineered cells that are introduced into blastocysts. These procedures for manipulation of ES cells and the construction of mice are technically demanding and most efficiently performed in a dedicated core laboratory. All of these alleles and technologies have been successfully implemented by the Core Directors, and many of the specific innovations were developed through prior support of this Project.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Heart, Lung, and Blood Institute (NHLBI)
Type
Research Program Projects (P01)
Project #
5P01HL049953-15
Application #
7464621
Study Section
Project Start
2007-06-01
Project End
2008-05-31
Budget Start
2007-06-01
Budget End
2008-05-31
Support Year
15
Fiscal Year
2007
Total Cost
$224,402
Indirect Cost

  Institution

Name
Texas A&M University
Department
Type
DUNS #
835607441
City
College Station
State
TX
Country
United States
Zip Code
77845

  Publications

Liu, Yu; Kaneda, Ruri; Leja, Thomas W et al. (2014) Hhex and Cer1 mediate the Sox17 pathway for cardiac mesoderm formation in embryonic stem cells. Stem Cells 32:1515-26
Zeve, Daniel; Seo, Jin; Suh, Jae Myoung et al. (2012) Wnt signaling activation in adipose progenitors promotes insulin-independent muscle glucose uptake. Cell Metab 15:492-504
Verzi, Michael P; Stanfel, Monique N; Moses, Kelvin A et al. (2009) Role of the homeodomain transcription factor Bapx1 in mouse distal stomach development. Gastroenterology 136:1701-10
Shah, Viraj R; Koster, Maranke I; Roop, Dennis R et al. (2007) Double-inducible gene activation system for caspase 3 and 9 in epidermis. Genesis 45:194-9
Niu, Zhivy; Li, Ankang; Zhang, Shu X et al. (2007) Serum response factor micromanaging cardiogenesis. Curr Opin Cell Biol 19:618-27
Chang, Jiang; Xie, Min; Shah, Viraj R et al. (2006) Activation of Rho-associated coiled-coil protein kinase 1 (ROCK-1) by caspase-3 cleavage plays an essential role in cardiac myocyte apoptosis. Proc Natl Acad Sci U S A 103:14495-500
Zhang, Ying-Min; Bo, Jacqueline; Taffet, George E et al. (2006) Targeted deletion of ROCK1 protects the heart against pressure overload by inhibiting reactive fibrosis. FASEB J 20:916-25
Ilagan, Roger; Abu-Issa, Radwan; Brown, Doris et al. (2006) Fgf8 is required for anterior heart field development. Development 133:2435-45
Barron, Matthew R; Belaguli, Narasimhaswamy S; Zhang, Shu Xiang et al. (2005) Serum response factor, an enriched cardiac mesoderm obligatory factor, is a downstream gene target for Tbx genes. J Biol Chem 280:11816-28
Zhang, Shu Xing; Garcia-Gras, Eduardo; Wycuff, Diane R et al. (2005) Identification of direct serum-response factor gene targets during Me2SO-induced P19 cardiac cell differentiation. J Biol Chem 280:19115-26

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