Abstract

The Vector Core is designed to facilitate gene therapy research and has been an active part of this PPG? throughout its funding. It has met and exceeded the specific aims during prior funding periods. This shared? facility has allowed consolidation of resources and promoted interactions among the gene therapy? investigators at IU. While the Core initially provided support for retroviral gene transfer, it has been heavily? invovled with the development of lentiviral vectors for PPG investigators.? The core will continue to provide PPG investigators with the following services: (1) Access to plasmid, cell? lines, and other reagents used in the generation of gene transfer vectors, (2) Generation and? characterization of retroviral and lentiviral vectors for preclinical work, (3) Molecular Diagnostics Services,? and (4) Access to BL3 Facilities and assistance with regulatory requirements. The objectives include new? services such as LAM-PCR and production of foamy viral vectors.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Heart, Lung, and Blood Institute (NHLBI)
Type
Research Program Projects (P01)
Project #
5P01HL053586-14
Application #
7642434
Study Section
Heart, Lung, and Blood Initial Review Group (HLBP)
Project Start
Project End
Budget Start
2008-07-01
Budget End
2009-06-30
Support Year
14
Fiscal Year
2008
Total Cost
$156,667
Indirect Cost

  Institution

Name
Indiana University-Purdue University at Indianapolis
Department
Type
DUNS #
603007902
City
Indianapolis
State
IN
Country
United States
Zip Code
46202

  Publications

Stein, Stefan; Scholz, Simone; Schwäble, Joachim et al. (2013) From bench to bedside: preclinical evaluation of a self-inactivating gammaretroviral vector for the gene therapy of X-linked chronic granulomatous disease. Hum Gene Ther Clin Dev 24:86-98
Song, Liujiang; Kauss, M Ariel; Kopin, Etana et al. (2013) Optimizing the transduction efficiency of capsid-modified AAV6 serotype vectors in primary human hematopoietic stem cells in vitro and in a xenograft mouse model in vivo. Cytotherapy 15:986-98
Liu, Ying; Ballman, Kimberly; Li, Deqiang et al. (2012) Impaired function of Fanconi anemia type C-deficient macrophages. J Leukoc Biol 91:333-40
Hawkins, Troy B; Dantzer, Jessica; Peters, Brandon et al. (2011) Identifying viral integration sites using SeqMap 2.0. Bioinformatics 27:720-2
Ou, Xuan; Chae, Hee-Don; Wang, Rui-Hong et al. (2011) SIRT1 deficiency compromises mouse embryonic stem cell hematopoietic differentiation, and embryonic and adult hematopoiesis in the mouse. Blood 117:440-50
Grez, Manuel; Reichenbach, Janine; Schwäble, Joachim et al. (2011) Gene therapy of chronic granulomatous disease: the engraftment dilemma. Mol Ther 19:28-35
Rohrabaugh, Sara L; Campbell, Timothy B; Hangoc, Giao et al. (2011) Ex vivo rapamycin treatment of human cord blood CD34+ cells enhances their engraftment of NSG mice. Blood Cells Mol Dis 46:318-20
Broxmeyer, Hal E; Lee, Man-Ryul; Hangoc, Giao et al. (2011) Hematopoietic stem/progenitor cells, generation of induced pluripotent stem cells, and isolation of endothelial progenitors from 21- to 23.5-year cryopreserved cord blood. Blood 117:4773-7
Rohrabaugh, Sara L; Hangoc, Giao; Kelley, Mark R et al. (2011) Mad2 haploinsufficiency protects hematopoietic progenitor cells subjected to cell-cycle stress in vivo and to inhibition of redox function of Ape1/Ref-1 in vitro. Exp Hematol 39:415-23
Liu, Ying; Timani, Khalid; Mantel, Charlie et al. (2011) TIP110/p110nrb/SART3/p110 regulation of hematopoiesis through CMYC. Blood 117:5643-51

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