Abstract

The function of Core B is to provide human and immune cell phenotyping for each project that will be critical to the translation of mechanistic findings into the human model. The effects of immune cell variations on atherosclerosis in humans represents a poody understood area of atherogenesis and possible atheroprotection. The goal of the Human Phenotyping and Immune Cell Core (Core B) is to provide the resources necessary for translation of novel immune mechanisms of atherosclerosis that are well defined in murine models into the human model. Specifically, we will provide well-defined phenotypes of atherosclerosis burden and risk in patients with and without type 2 diabetes, genotyping analysis, and phenotypic descriptions of immune cells in humans using flow cytometry. Core B will work with each of the three projects to provide in patients, with and without Type 2 diabetes, well-defined phenotypes of atherosclerosis burden and risk using the Framingham risk Score and carotid intima-media thickness (projects 1-3);serum and plasma measurement of traditional atherosclerotic risk factors (projects 1-3);genotyping of single nucleotide polymorphisms related to atherosclerotic mechanisms (project 3);isolation and FACS analysis of immune cells (projects 1,3);and collection and shipping of serum and plasma for analysis by individual projects (projects 1-3).

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Heart, Lung, and Blood Institute (NHLBI)
Type
Research Program Projects (P01)
Project #
2P01HL055798-16A1
Application #
8396707
Study Section
Special Emphasis Panel (ZHL1-CSR-A (M1))
Project Start
Project End
Budget Start
2012-08-01
Budget End
2013-07-31
Support Year
16
Fiscal Year
2012
Total Cost
$375,435
Indirect Cost
$18,500

  Institution

Name
La Jolla Institute
Department
Type
DUNS #
603880287
City
La Jolla
State
CA
Country
United States
Zip Code
92037

  Publications

Jeong, Se-Jin; Kim, Sinai; Park, Jong-Gil et al. (2018) Prdx1 (peroxiredoxin 1) deficiency reduces cholesterol efflux via impaired macrophage lipophagic flux. Autophagy 14:120-133
Gao, Chuan; Tabb, Keri L; Dimitrov, Latchezar M et al. (2018) Exome Sequencing Identifies Genetic Variants Associated with Circulating Lipid Levels in Mexican Americans: The Insulin Resistance Atherosclerosis Family Study (IRASFS). Sci Rep 8:5603
Gaddis, Dalia E; Padgett, Lindsey E; Wu, Runpei et al. (2018) Apolipoprotein AI prevents regulatory to follicular helper T cell switching during atherosclerosis. Nat Commun 9:1095
Que, Xuchu; Hung, Ming-Yow; Yeang, Calvin et al. (2018) Oxidized phospholipids are proinflammatory and proatherogenic in hypercholesterolaemic mice. Nature 558:301-306
Senders, Max L; Que, Xuchu; Cho, Young Seok et al. (2018) PET/MR Imaging of Malondialdehyde-Acetaldehyde Epitopes With a Human Antibody Detects Clinically Relevant Atherothrombosis. J Am Coll Cardiol 71:321-335
Capoulade, Romain; Chan, Kwan L; Mathieu, Patrick et al. (2017) Autoantibodies and immune complexes to oxidation-specific epitopes and progression of aortic stenosis: Results from the ASTRONOMER trial. Atherosclerosis 260:1-7
van Capelleveen, Julian C; Bernelot Moens, Sophie J; Yang, Xiaohong et al. (2017) Apolipoprotein C-III Levels and Incident Coronary Artery Disease Risk: The EPIC-Norfolk Prospective Population Study. Arterioscler Thromb Vasc Biol 37:1206-1212
Pechlaner, Raimund; Tsimikas, Sotirios; Yin, Xiaoke et al. (2017) Very-Low-Density Lipoprotein-Associated Apolipoproteins Predict Cardiovascular Events and Are Lowered by Inhibition of APOC-III. J Am Coll Cardiol 69:789-800
Lee, Sang-Rok; Prasad, Anand; Choi, Yun-Seok et al. (2017) LPA Gene, Ethnicity, and Cardiovascular Events. Circulation 135:251-263
Kamstrup, Pia R; Hung, Ming-Yow; Witztum, Joseph L et al. (2017) Oxidized Phospholipids and Risk of Calcific Aortic Valve Disease: The Copenhagen General Population Study. Arterioscler Thromb Vasc Biol 37:1570-1578

Showing the most recent 10 out of 217 publications