The purpose of Core A is to provide assistance with state-of-the-art measurements of reactive oxygen species (ROS) and nitric oxide (NO) in vascular cells and tissues for the participants of the PPG. CORE A will assist with preparation and processing of various biological samples for analysis using Electron Spin Resonance (ESR) spectroscopy. ESR spectroscopy with a spin trapping technique is one of the most direct and unequivocal methods for high sensitivity quantitative detection of reactive oxygen species even in strongly scattering media using minimal amounts of biological material. This Core facility has existed since 1999 and has supported studies of superoxide radical and nitric oxide in vascular cells in several publications from Drs. Griendling and Harrison. This facility will significantly enhance the work of the individual labs and will clearly improve collaborations between participants of the PPG project. Specifically, the Electron Spin Resonance Core will: 1. Offer expertise, facilities, and training for individuals in performing measurements of ROS and NO using ESR spectroscopy. 2. Provide assistance with state-of-the-art in vitro and ex vivo measurements of ROS and nitric oxide in vascular cells and tissues for participants of the PPG. 3. Develop techniques and refine existing methods for measurement of ROS and NO in vascular tissues.
| Yanes, Rolando E; Gustafson, Claire E; Weyand, Cornelia M et al. (2017) Lymphocyte generation and population homeostasis throughout life. Semin Hematol 54:33-38 |
| Kim, C; Fang, F; Weyand, C M et al. (2017) The life cycle of a T cell after vaccination - where does immune ageing strike? Clin Exp Immunol 187:71-81 |
| Foss, Jason D; Kirabo, Annet; Harrison, David G (2017) Do high-salt microenvironments drive hypertensive inflammation? Am J Physiol Regul Integr Comp Physiol 312:R1-R4 |
| Loperena, Roxana; Harrison, David G (2017) Oxidative Stress and Hypertensive Diseases. Med Clin North Am 101:169-193 |
| Weyand, Cornelia M; Zeisbrich, Markus; Goronzy, Jörg J (2017) Metabolic signatures of T-cells and macrophages in rheumatoid arthritis. Curr Opin Immunol 46:112-120 |
| Weyand, Cornelia M; Goronzy, Jörg J (2016) Aging of the Immune System. Mechanisms and Therapeutic Targets. Ann Am Thorac Soc 13 Suppl 5:S422-S428 |
| Wu, Jing; Montaniel, Kim Ramil C; Saleh, Mohamed A et al. (2016) Origin of Matrix-Producing Cells That Contribute to Aortic Fibrosis in Hypertension. Hypertension 67:461-8 |
| Wenzel, Ulrich; Turner, Jan Eric; Krebs, Christian et al. (2016) Immune Mechanisms in Arterial Hypertension. J Am Soc Nephrol 27:677-86 |
| Montaniel, Kim Ramil C; Harrison, David G (2016) Is Hypertension a Bone Marrow Disease? Circulation 134:1369-1372 |
| Wen, Zhenke; Shimojima, Yasuhiro; Shirai, Tsuyoshi et al. (2016) NADPH oxidase deficiency underlies dysfunction of aged CD8+ Tregs. J Clin Invest 126:1953-67 |
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