The purpose of this core unit is to utilize discarded vascular material at Brigham and Women's Hospital in order to provide primary human vascular cells for Projects 1, 2, and 3 as well as rat cardiomyocytes for Project 4. Through a dedicated cell culture technician, we will obtain human saphenous and human aortic tissue (excess tissue from orthotopic hear and Women's Hospital and provide cells to the projects at MIT; this will be much more efficient than individual procurement of tissues by investigators at MIT. Dr. Lee's laboratory is in the Thorn Research Building, attached to the main hospital building and allowing rapid access to these discarded tissues.

Agency
National Institute of Health (NIH)
Institute
National Heart, Lung, and Blood Institute (NHLBI)
Type
Research Program Projects (P01)
Project #
1P01HL064858-01A1
Application #
6326715
Study Section
Heart, Lung, and Blood Program Project Review Committee (HLBP)
Project Start
2001-04-01
Project End
2006-03-31
Budget Start
Budget End
Support Year
1
Fiscal Year
2001
Total Cost
Indirect Cost
Name
Massachusetts Institute of Technology
Department
Type
DUNS #
City
Cambridge
State
MA
Country
United States
Zip Code
02139
Rahim, Nur Aida Adbul; Pelet, Serge; Kamm, Roger D et al. (2012) Methodological considerations for global analysis of cellular FLIM/FRET measurements. J Biomed Opt 17:026013
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Jonas, Maxine; Huang, Hayden; Kamm, Roger D et al. (2008) Fast fluorescence laser tracking microrheometry. I: instrument development. Biophys J 94:1459-69
Jonas, Maxine; Huang, Hayden; Kamm, Roger D et al. (2008) Fast fluorescence laser tracking microrheometry, II: quantitative studies of cytoskeletal mechanotransduction. Biophys J 95:895-909
Ferrer, Jorge M; Lee, Hyungsuk; Chen, Jiong et al. (2008) Measuring molecular rupture forces between single actin filaments and actin-binding proteins. Proc Natl Acad Sci U S A 105:9221-6
Previte, Michael J R; Pelet, Serge; Kim, Ki Hean et al. (2008) Spectrally resolved fluorescence correlation spectroscopy based on global analysis. Anal Chem 80:3277-84
Lee, S E; Chunsrivirot, S; Kamm, R D et al. (2008) Molecular dynamics study of talin-vinculin binding. Biophys J 95:2027-36
Jonas, Maxine; So, Peter T C; Huang, Hayden (2008) Cell mechanics at multiple scales. Methods Enzymol 443:177-98
Norton, G V; Novarini, J C (2007) Modeling ultrasonic transient scattering from biological tissues including their dispersive properties directly in the time domain. Mol Cell Biomech 4:75-85
Su, Judith; Jiang, Xingyu; Welsch, Roy et al. (2007) Geometric confinement influences cellular mechanical properties I -- adhesion area dependence. Mol Cell Biomech 4:87-104

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