Abstract

The Pathology/Animal Core will provide the Program Project uniform processing and staining of cells and tissues of microscopy. The Core will offer expert immunohistochemistry on light and transmission electron microscopy (EM) from the 4 Projects. More importantly, it will provide an experienced pathologist in the field of inflammation and repair, who can compare and integrate the pathological findings in all of the models. Most of the immunopathological methods employed are common to all of the projects so that a Core laboratory will provide uniform methods and interpretation. The central processing of tissue will result in cost savings, quality control and comparability between the results of the projects. The continual review of all of the histological findings by a single pathologist will ensure that both common and unique aspects of pathological results from different projects are appreciated immediately. The Animal portion of the Core will provide preparation and surgery for the intact animal models of myocardial ischemia/reperfusion used in Projects 1, 3, and 4. These models will include acute and/or chronic studies in mice, rats, and dogs. Each model has been chosen for a specific scientific purpose and will be supervised by a physiologists who had previous experience with each.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Heart, Lung, and Blood Institute (NHLBI)
Type
Research Program Projects (P01)
Project #
5P01HL065608-02
Application #
6494015
Study Section
Heart, Lung, and Blood Initial Review Group (HLBP)
Project Start
2001-08-01
Project End
2002-07-31
Budget Start
Budget End
Support Year
2
Fiscal Year
2001
Total Cost
$297,094
Indirect Cost

  Institution

Name
Johns Hopkins University
Department
Type
DUNS #
045911138
City
Baltimore
State
MD
Country
United States
Zip Code
21218

  Publications

Velayutham, Murugesan; Hemann, Craig F; Cardounel, Arturo J et al. (2016) Sulfite Oxidase Activity of Cytochrome c: Role of Hydrogen Peroxide. Biochem Biophys Rep 5:96-104
Xie, Lin; Talukder, M A Hassan; Sun, Jian et al. (2015) Liposomal tetrahydrobiopterin preserves eNOS coupling in the post-ischemic heart conferring in vivo cardioprotection. J Mol Cell Cardiol 86:14-22
Long 3rd, Victor P; Bonilla, Ingrid M; Vargas-Pinto, Pedro et al. (2015) Heart failure duration progressively modulates the arrhythmia substrate through structural and electrical remodeling. Life Sci 123:61-71
Reyes, Levy A; Boslett, James; Varadharaj, Saradhadevi et al. (2015) Depletion of NADP(H) due to CD38 activation triggers endothelial dysfunction in the postischemic heart. Proc Natl Acad Sci U S A 112:11648-53
Joddar, Binata; Firstenberg, Michael S; Reen, Rashmeet K et al. (2015) Arterial levels of oxygen stimulate intimal hyperplasia in human saphenous veins via a ROS-dependent mechanism. PLoS One 10:e0120301
Zheng, Xiaoxu; Zu, Lingyun; Becker, Lewis et al. (2014) Ischemic preconditioning inhibits mitochondrial permeability transition pore opening through the PTEN/PDE4 signaling pathway. Cardiology 129:163-73
Moldovan, Nicanor I; Anghelina, Mirela; Varadharaj, Saradhadevi et al. (2014) Reoxygenation-derived toxic reactive oxygen/nitrogen species modulate the contribution of bone marrow progenitor cells to remodeling after myocardial infarction. J Am Heart Assoc 3:e000471
Huang, Jie; Huffman, Jennifer E; Yamakuchi, Munekazu et al. (2014) Genome-wide association study for circulating tissue plasminogen activator levels and functional follow-up implicates endothelial STXBP5 and STX2. Arterioscler Thromb Vasc Biol 34:1093-101
Chen, Yeong-Renn; Zweier, Jay L (2014) Cardiac mitochondria and reactive oxygen species generation. Circ Res 114:524-37
Tong, Jianjing; Zweier, Joseph R; Huskey, Rachael L et al. (2014) Effect of temperature, pH and heme ligands on the reduction of Cygb(Fe(3+)) by ascorbate. Arch Biochem Biophys 554:1-5

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