Core E will provide all histological, histochemical, electron microscopic and immunohistochemical techniquesrequired by the four projects. In addition core E will provide histopathological interpretations and quantitativeimage analyses on tissues and cultured cells as required by the projects. Two pathologists and ahistotechnician comprise the core personnel. The two pathologists work closely with the project directors toassure the quality of the experimental results. The core operates 5 microscopes equipped for fluorescenceand bright field microscopy and digital imaging for use of the core personnel and researchers from theprojects. The core also maintains two confocal microscopes for detailed fluorescence studies. The repertoryof stains includes H&E, trichrome, methenamine silver and picric acid Sirius red. The most often employedimmunostains are for Kit and Ki-67. In addition the core provides a wide range of immunohistochemicalprocedures using diverse antibodies. TUNEL assays for presumptive apoptotic cells are routine in the core.Quantitative image analytic procedures include cell frequency, collagen assay by direct a real measurementand point counting for volume density measurements on gross, light microscopic and electron microscopicimages.
Showing the most recent 10 out of 196 publications
