Abstract

RNA Expression Profiling Core. A comprehensive analysis of RNA expression in distinct cell types has been revolutionized by the use of DNA microarray analysis. This methodology can assess the expression patterns of thousands of genes in quantitative terms with small amounts of cells or tissue. This analysis seeks to develop genetic markers for the models under study in each aim in the in vitro and in vivo analyses that form the basis of this proposal. Identification of such markers can then be used to facilitate the validation of the hypotheses of these models. The use of RNA markers should be a reliable method for defining cellular expression profiles. Once a set of markers has been identified these sequences can then be used by additional methods (e.g., in situ hybridization) to further test the hypotheses. Core E will work with investigators in Projects 1 and 2 to identify markers from cultured cells and from experimentally manipulated vascular tissues that can be compared with RNA prepared from human GA and control arteries in Core D.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Heart, Lung, and Blood Institute (NHLBI)
Type
Research Program Projects (P01)
Project #
5P01HL070295-02
Application #
6659336
Study Section
Special Emphasis Panel (ZAI1)
Project Start
2002-09-01
Project End
2003-08-31
Budget Start
2002-09-01
Budget End
2003-08-31
Support Year
2
Fiscal Year
2002
Total Cost
$177,467
Indirect Cost

  Institution

Name
Yale University
Department
Type
DUNS #
082359691
City
New Haven
State
CT
Country
United States
Zip Code
06520

  Publications

Kraehling, Jan R; Chidlow, John H; Rajagopal, Chitra et al. (2016) Genome-wide RNAi screen reveals ALK1 mediates LDL uptake and transcytosis in endothelial cells. Nat Commun 7:13516
Siragusa, Mauro; Fröhlich, Florian; Park, Eon Joo et al. (2015) Stromal cell-derived factor 2 is critical for Hsp90-dependent eNOS activation. Sci Signal 8:ra81
Lee, Monica Y; Luciano, Amelia K; Ackah, Eric et al. (2014) Endothelial Akt1 mediates angiogenesis by phosphorylating multiple angiogenic substrates. Proc Natl Acad Sci U S A 111:12865-70
Pober, Jordan S; Jane-wit, Dan; Qin, Lingfeng et al. (2014) Interacting mechanisms in the pathogenesis of cardiac allograft vasculopathy. Arterioscler Thromb Vasc Biol 34:1609-14
Park, Eon Joo; Grabi?ska, Kariona A; Guan, Ziqiang et al. (2014) Mutation of Nogo-B receptor, a subunit of cis-prenyltransferase, causes a congenital disorder of glycosylation. Cell Metab 20:448-57
Wang, Chen; Yi, Tai; Qin, Lingfeng et al. (2013) Rapamycin-treated human endothelial cells preferentially activate allogeneic regulatory T cells. J Clin Invest 123:1677-93
Yi, Tai; Fogal, Birgit; Hao, Zhengrong et al. (2012) Reperfusion injury intensifies the adaptive human T cell alloresponse in a human-mouse chimeric artery model. Arterioscler Thromb Vasc Biol 32:353-60
Zhang, Jiasheng; Razavian, Mahmoud; Tavakoli, Sina et al. (2012) Molecular imaging of vascular endothelial growth factor receptors in graft arteriosclerosis. Arterioscler Thromb Vasc Biol 32:1849-55
Marin, Ethan P; Derakhshan, Behrad; Lam, TuKiet T et al. (2012) Endothelial cell palmitoylproteomic identifies novel lipid-modified targets and potential substrates for protein acyl transferases. Circ Res 110:1336-44
Forstermann, Ulrich; Sessa, William C (2012) Nitric oxide synthases: regulation and function. Eur Heart J 33:829-37, 837a-837d

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