Abstract

The Fluorescence Microscopy and Imaging Core (Core B) will provide technical expertise in Fluorescence Microscopy and quantitative image processing applications, including optical sectioning and three- dimensional reconstruction, fluorescence resonance energy transfer (FRET) and quantitative live-cell fluorescence imaging, to members of the program. The core will be located at the Center for Biomedical Imaging Technology (CBIT) at UCHC. CBIT is a well-established research center with a focus on the development and application for fluorescence imaging technology and the use of computational modeling in cell biological research. Dr. AnncOWAN, Deputy Director of the Center for Biomedical Imaging Technology (CBIT) will direct the Core. Services provided by the core will include access to and technical assistance with state of the art instrumentation and software, and expertise in experimental design and data interpretation for fluorescence imaging applications. Microscopy facilities available at the core include 4 confocal microscopes, 2 with non-linear (multi-photon) capabilities, 2 widefield (traditional) fluorescence microscope workstations equipped with high sensitivity CCD cameras, and a host of image processing, three-dimensional rendering, and presentation software.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Heart, Lung, and Blood Institute (NHLBI)
Type
Research Program Projects (P01)
Project #
1P01HL070694-01
Application #
6633631
Study Section
Heart, Lung, and Blood Initial Review Group (HLBP)
Project Start
2002-07-01
Project End
2007-06-30
Budget Start
2002-07-01
Budget End
2003-06-30
Support Year
1
Fiscal Year
2002
Total Cost
$1
Indirect Cost

  Institution

Name
University of Connecticut
Department
Type
DUNS #
City
Farmington
State
CT
Country
United States
Zip Code
06030

  Publications

Gerber, Claire; Harel, Miriam; Lynch, Miranda L et al. (2016) Proximal tubule proteins are significantly elevated in bladder urine of patients with ureteropelvic junction obstruction and may represent novel biomarkers: A pilot study. J Pediatr Urol 12:120.e1-7
Harel, Miriam; Ferrer, Fernando A; Shapiro, Linda H et al. (2016) Future directions in risk stratification and therapy for advanced pediatric genitourinary rhabdomyosarcoma. Urol Oncol 34:103-15
Conway, Rebecca E; Rojas, Camilo; Alt, Jesse et al. (2016) Prostate-specific membrane antigen (PSMA)-mediated laminin proteolysis generates a pro-angiogenic peptide. Angiogenesis 19:487-500
Galvani, Sylvain; Sanson, Marie; Blaho, Victoria A et al. (2015) HDL-bound sphingosine 1-phosphate acts as a biased agonist for the endothelial cell receptor S1P1 to limit vascular inflammation. Sci Signal 8:ra79
Ghosh, Mallika; Subramani, Jaganathan; Rahman, M Mamunur et al. (2015) CD13 restricts TLR4 endocytic signal transduction in inflammation. J Immunol 194:4466-76
Blaho, Victoria A; Galvani, Sylvain; Engelbrecht, Eric et al. (2015) HDL-bound sphingosine-1-phosphate restrains lymphopoiesis and neuroinflammation. Nature 523:342-6
Ghosh, Mallika; Gerber, Claire; Rahman, M Mamunur et al. (2014) Molecular mechanisms regulating CD13-mediated adhesion. Immunology 142:636-47
Blaho, Victoria A; Hla, Timothy (2014) An update on the biology of sphingosine 1-phosphate receptors. J Lipid Res 55:1596-608
Rahman, M Mamunur; Ghosh, Mallika; Subramani, Jaganathan et al. (2014) CD13 regulates anchorage and differentiation of the skeletal muscle satellite stem cell population in ischemic injury. Stem Cells 32:1564-77
Xiong, Yuquan; Lee, Hyuek Jong; Mariko, Boubacar et al. (2013) Sphingosine kinases are not required for inflammatory responses in macrophages. J Biol Chem 288:32563-73

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